Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 175031..176654 Replicon plasmid pEL-ars2
Accession NZ_CP094843
Organism Enterobacter ludwigii strain Sb-9

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag MT488_RS24945 Protein ID WP_000088044.1
Coordinates 175329..176654 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag MT488_RS24940 Protein ID WP_012477377.1
Coordinates 175031..175324 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT488_RS24925 (MT488_24925) 170307..170711 - 405 Protein_160 recombinase family protein -
MT488_RS24930 (MT488_24930) 170879..173865 + 2987 Protein_161 Tn3 family transposase -
MT488_RS24935 (MT488_24935) 173944..174630 + 687 WP_244048037.1 hypothetical protein -
MT488_RS24940 (MT488_24940) 175031..175324 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
MT488_RS24945 (MT488_24945) 175329..176654 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
MT488_RS24950 (MT488_24950) 176715..176921 + 207 WP_000134171.1 hypothetical protein -
MT488_RS24955 (MT488_24955) 177022..177432 + 411 WP_000985909.1 hypothetical protein -
MT488_RS24960 (MT488_24960) 177445..178260 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
MT488_RS24965 (MT488_24965) 178513..178938 + 426 WP_000278471.1 hypothetical protein -
MT488_RS24970 (MT488_24970) 179487..179795 + 309 WP_001224686.1 hypothetical protein -
MT488_RS24975 (MT488_24975) 179811..180668 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-9 - 1..210249 210249


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T240645 WP_000088044.1 NZ_CP094843:175329-176654 [Enterobacter ludwigii]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T240645 NZ_LT604072:c4464411-4464277 [Xanthomonas translucens pv. translucens DSM 18974]
ATGAAGCGTACTTTCGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGTCGGGCTGCAACACGGTTGCCGGTGC
CGGCAAGGACGTGAAGAGCGCCGGCGAGAAGGTCGAAGACGCCGCCAAGAACTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT240645 WP_012477377.1 NZ_CP094843:175031-175324 [Enterobacter ludwigii]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT240645 NZ_LT604072:c4464662-4464510 [Xanthomonas translucens pv. translucens DSM 18974]
ATGAAGCGACTGTTCACCTTGACGATGCTGACCCTGTTTTGTGCAGGCATGCTGACCGGCTGCAACACCATGGCCGGCGC
CGGTAAGGACATGCAGAAGGCCGGCGATAAGGTGGAAACCAAGGCCGACGACTGTAAAGACGGCAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References