Detailed information of TA system TA240643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 2646..3289
• Replicon: plasmid pEL-ars1
• Accession: NZ_CP094842
• Organism: Enterobacter ludwigii strain Sb-9

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MT488_RS23370
• Protein ID: WP_148576981.1
• Coordinates: 2873..3289 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: MT488_RS23365
• Protein ID: WP_148576982.1
• Coordinates: 2646..2876 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT488_RS23355 (1)	1..1011	+	1011	WP_148576983.1	RepB family plasmid replication initiator protein	-
MT488_RS23360 (1266)	1266..1652	-	387	Protein_1	tyrosine-type recombinase/integrase	-
MT488_RS23365 (2646)	2646..2876	+	231	WP_148576982.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MT488_RS23370 (2873)	2873..3289	+	417	WP_148576981.1	type II toxin-antitoxin system VapC family toxin	Toxin
MT488_RS23375 (4068)	4068..5267	+	1200	WP_094833309.1	cation:proton antiporter	-
MT488_RS23380 (5319)	5319..5738	+	420	WP_050484447.1	universal stress protein	-
MT488_RS23385 (5977)	5977..7515	+	1539	WP_148576980.1	DNA mismatch repair protein MutS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-10	-	1..145699	145699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15132.66 Da
• Isoelectric Point: 8.5291

>T240643 WP_148576981.1 NZ_CP094842:2873-3289 [Enterobacter ludwigii]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASLRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRLVGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLENWVK

Nucleotide

• Length: 417 bp

>T240643 NZ_LT604072:3582088-3582339 [Xanthomonas translucens pv. translucens DSM 18974]
ATGGACACCATCACCTATAGCGCTGCACGCGCCGCCCTTGCGGACACCATGGAGCGTGTCGTCAACAATCACGAGCCGGT
GATCATCACCCGCAGCCGCGAGCAATCCGTGGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTACC
TGCTGCGCAGCCCGAAGAGCGCGCAGCGCCTGCTGGAATCCATCGCCCAGCTAGAGGCCGGTCGCGGCAAGGCACGGGAC
CTGGCCGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8766.86 Da
• Isoelectric Point: 4.2917

>AT240643 WP_148576982.1 NZ_CP094842:2646-2876 [Enterobacter ludwigii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT240643 NZ_LT604072:3582336-3582593 [Xanthomonas translucens pv. translucens DSM 18974]
GTGACGCTTCAGTTCTCGGACAATGCCTGGGAGGACGACCTCTACTGGCAGCAGACCGACAAGAAGATGCTCAAGCGCAT
CAACGACCTGATCAAAGCCATTCAACGCGATCCCTTCCAGGGCGTCGGCAAGCCAGAACGGCTACGCCATGCCTTGGCCG
GCTATTGGTCGCGGCGCATCAACGACGAACACCGCATCGTCTACAAGGTCGAGGCGGGCGTCCTACTGATCGCGCAGGTG
CGGTATCACTACGCGTAG