Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1031428..1032048 Replicon chromosome
Accession NZ_CP094841
Organism Enterobacter ludwigii strain Sb-9

Toxin (Protein)


Gene name Hha Uniprot ID G8LPY2
Locus tag MT488_RS04895 Protein ID WP_014168902.1
Coordinates 1031428..1031646 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID W0BRV6
Locus tag MT488_RS04900 Protein ID WP_020885187.1
Coordinates 1031674..1032048 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MT488_RS04865 (MT488_04865) 1027439..1027699 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
MT488_RS04870 (MT488_04870) 1027702..1027842 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MT488_RS04875 (MT488_04875) 1027839..1028549 - 711 WP_244047719.1 GNAT family protein -
MT488_RS04880 (MT488_04880) 1028650..1030110 + 1461 WP_244047720.1 PLP-dependent aminotransferase family protein -
MT488_RS04885 (MT488_04885) 1030082..1030549 - 468 WP_032677533.1 YlaC family protein -
MT488_RS04890 (MT488_04890) 1030667..1031218 - 552 WP_020885188.1 maltose O-acetyltransferase -
MT488_RS04895 (MT488_04895) 1031428..1031646 - 219 WP_014168902.1 HHA domain-containing protein Toxin
MT488_RS04900 (MT488_04900) 1031674..1032048 - 375 WP_020885187.1 Hha toxicity modulator TomB Antitoxin
MT488_RS04905 (MT488_04905) 1032560..1035706 - 3147 WP_014168904.1 multidrug efflux RND transporter permease subunit AcrB -
MT488_RS04910 (MT488_04910) 1035729..1036922 - 1194 WP_020885186.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8654.04 Da        Isoelectric Point: 8.9107

>T240633 WP_014168902.1 NZ_CP094841:c1031646-1031428 [Enterobacter ludwigii]
MSEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWRFVR

Download         Length: 219 bp

>T240633 NZ_LT601384:c3603664-3603350 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8886

>AT240633 WP_020885187.1 NZ_CP094841:c1032048-1031674 [Enterobacter ludwigii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFINVSRANPVSLSC

Download         Length: 375 bp

>AT240633 NZ_LT601384:c3603353-3602937 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BM33

Antitoxin

Source ID Structure
AlphaFold DB A0A5C1BX74

References