Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3180886..3181517 Replicon chromosome
Accession NC_012943
Organism Mycobacterium tuberculosis KZN 1435

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag TBMG_RS14990 Protein ID WP_003898728.1
Coordinates 3181206..3181517 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag TBMG_RS14985 Protein ID WP_003405836.1
Coordinates 3180886..3181206 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBMG_RS14955 3176082..3176720 + 639 WP_003898730.1 lipid droplet-associated protein -
TBMG_RS14960 3176717..3177964 + 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
TBMG_RS14965 3177954..3178211 + 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
TBMG_RS14970 3178221..3179333 + 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
TBMG_RS21560 3179351..3180876 - 1526 Protein_2936 carboxylesterase/lipase family protein -
TBMG_RS14985 3180886..3181206 + 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
TBMG_RS14990 3181206..3181517 + 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
TBMG_RS14995 3181629..3182786 + 1158 WP_003898727.1 AI-2E family transporter -
TBMG_RS15000 3182793..3183437 - 645 WP_003915494.1 DUF4245 domain-containing protein -
TBMG_RS15005 3183493..3184581 + 1089 WP_003898726.1 class II fructose-bisphosphatase -
TBMG_RS15010 3184612..3186036 + 1425 WP_003405805.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T24063 WP_003898728.1 NC_012943:3181206-3181517 [Mycobacterium tuberculosis KZN 1435]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T24063 NC_012943:3181206-3181517 [Mycobacterium tuberculosis KZN 1435]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT24063 WP_003405836.1 NC_012943:3180886-3181206 [Mycobacterium tuberculosis KZN 1435]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT24063 NC_012943:3180886-3181206 [Mycobacterium tuberculosis KZN 1435]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References