Detailed information of TA system TA240628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1745825..1746354
• Replicon: chromosome
• Accession: NZ_CP094825
• Organism: Staphylococcus ureilyticus strain IVB6236

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A6N7XWP7
• Locus tag: MUA21_RS08660
• Protein ID: WP_019468010.1
• Coordinates: 1745825..1746187 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A921LYV8
• Locus tag: MUA21_RS08665
• Protein ID: WP_046207786.1
• Coordinates: 1746184..1746354 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA21_RS08640 (MUA21_08610)	1742857..1743627	-	771	WP_262561086.1	RNA polymerase sigma factor SigB	-
MUA21_RS08645 (MUA21_08615)	1743602..1744084	-	483	WP_107526309.1	anti-sigma B factor RsbW	-
MUA21_RS08650 (MUA21_08620)	1744086..1744412	-	327	WP_046207788.1	anti-sigma factor antagonist	-
MUA21_RS08655 (MUA21_08625)	1744481..1745482	-	1002	WP_046207787.1	PP2C family protein-serine/threonine phosphatase	-
MUA21_RS08660 (MUA21_08630)	1745825..1746187	-	363	WP_019468010.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA21_RS08665 (MUA21_08635)	1746184..1746354	-	171	WP_046207786.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA21_RS08670 (MUA21_08640)	1746442..1747590	-	1149	WP_064209856.1	alanine racemase	-
MUA21_RS08675 (MUA21_08645)	1747697..1748047	-	351	WP_046207784.1	holo-ACP synthase	-
MUA21_RS08680 (MUA21_08650)	1748082..1748573	-	492	WP_107526312.1	PH domain-containing protein	-
MUA21_RS08685 (MUA21_08655)	1748566..1750071	-	1506	WP_262561087.1	PH domain-containing protein	-
MUA21_RS08690 (MUA21_08660)	1750064..1750543	-	480	WP_073346060.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13595.69 Da
• Isoelectric Point: 9.9426

>T240628 WP_019468010.1 NZ_CP094825:c1746187-1745825 [Staphylococcus ureilyticus]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSEEKMQEVNNAIDISLGLHNIRNHRT

Nucleotide

• Length: 363 bp

>T240628 NZ_LT601384:2978240-2978518 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6332.00 Da
• Isoelectric Point: 4.0121

>AT240628 WP_046207786.1 NZ_CP094825:c1746354-1746184 [Staphylococcus ureilyticus]
MASFNQSRNHSIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYIISKNNNE

Nucleotide

• Length: 171 bp

>AT240628 NZ_LT601384:2977977-2978237 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A6N7XWP7