Detailed information of TA system TA024062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3172193..3172761
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: TBMG_RS14930
• Protein ID: WP_003405865.1
• Coordinates: 3172193..3172567 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: TBMG_RS14935
• Protein ID: WP_003405863.1
• Coordinates: 3172564..3172761 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS14895	3168606..3169313	+	708	Protein_2920	adenylate/guanylate cyclase domain-containing protein	-
TBMG_RS22550	3169265..3169474	-	210	WP_003911400.1	hypothetical protein	-
TBMG_RS14905	3169496..3170206	+	711	WP_003911399.1	hypothetical protein	-
TBMG_RS14910	3170221..3170544	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
TBMG_RS14915	3170787..3171062	+	276	WP_003405867.1	hypothetical protein	-
TBMG_RS14920	3170989..3171174	-	186	WP_003901093.1	hypothetical protein	-
TBMG_RS14925	3171292..3171990	-	699	WP_003898733.1	hypothetical protein	-
TBMG_RS14930	3172193..3172567	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBMG_RS14935	3172564..3172761	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBMG_RS14940	3172849..3173922	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
TBMG_RS14945	3173985..3174968	+	984	WP_003898731.1	hypothetical protein	-
TBMG_RS14950	3174985..3175992	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
TBMG_RS14955	3176082..3176720	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T24062 WP_003405865.1 NC_012943:c3172567-3172193 [Mycobacterium tuberculosis KZN 1435]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T24062 NC_012943:c3172567-3172193 [Mycobacterium tuberculosis KZN 1435]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT24062 WP_003405863.1 NC_012943:c3172761-3172564 [Mycobacterium tuberculosis KZN 1435]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT24062 NC_012943:c3172761-3172564 [Mycobacterium tuberculosis KZN 1435]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07