Detailed information of TA system TA024061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3027325..3028013
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: TBMG_RS14265
• Protein ID: WP_003406304.1
• Coordinates: 3027325..3027756 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: TBMG_RS14270
• Protein ID: WP_003406302.1
• Coordinates: 3027753..3028013 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS14240	3023047..3023316	+	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TBMG_RS14245	3023313..3023606	+	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
TBMG_RS14250	3023663..3024493	+	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
TBMG_RS14255	3024574..3025434	-	861	WP_003900301.1	hypothetical protein	-
TBMG_RS14260	3025614..3027302	+	1689	WP_003910308.1	PE family protein	-
TBMG_RS14265	3027325..3027756	-	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBMG_RS14270	3027753..3028013	-	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
TBMG_RS14275	3028089..3029078	-	990	WP_003406301.1	malate dehydrogenase	-
TBMG_RS14280	3029249..3030349	+	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
TBMG_RS14285	3030426..3031607	-	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
TBMG_RS14290	3031612..3032436	-	825	WP_003900298.1	trehalose ABC transporter permease SugB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T24061 WP_003406304.1 NC_012943:c3027756-3027325 [Mycobacterium tuberculosis KZN 1435]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T24061 NC_012943:c3027756-3027325 [Mycobacterium tuberculosis KZN 1435]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT24061 WP_003406302.1 NC_012943:c3028013-3027753 [Mycobacterium tuberculosis KZN 1435]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT24061 NC_012943:c3028013-3027753 [Mycobacterium tuberculosis KZN 1435]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6