Detailed information of TA system TA240597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 854845..855384
• Replicon: chromosome
• Accession: NZ_CP094816
• Organism: Mammaliicoccus sciuri strain IVB6231

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A418JBZ2
• Locus tag: MUA23_RS04125
• Protein ID: WP_048540867.1
• Coordinates: 855013..855384 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MUA23_RS04120
• Protein ID: WP_048540868.1
• Coordinates: 854845..855012 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA23_RS04095 (MUA23_04095)	850727..851206	+	480	WP_058591087.1	PH domain-containing protein	-
MUA23_RS04100 (MUA23_04100)	851199..852716	+	1518	WP_262657401.1	PH domain-containing protein	-
MUA23_RS04105 (MUA23_04105)	852706..853170	+	465	WP_075578106.1	PH domain-containing protein	-
MUA23_RS04110 (MUA23_04110)	853202..853564	+	363	WP_058591084.1	holo-ACP synthase	-
MUA23_RS04115 (MUA23_04115)	853612..854760	+	1149	WP_106417899.1	alanine racemase	-
MUA23_RS04120 (MUA23_04120)	854845..855012	+	168	WP_048540868.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA23_RS04125 (MUA23_04125)	855013..855384	+	372	WP_048540867.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA23_RS04130 (MUA23_04130)	855481..856485	+	1005	WP_106417900.1	PP2C family protein-serine/threonine phosphatase	-
MUA23_RS04135 (MUA23_04135)	856590..856913	+	324	WP_025904513.1	anti-sigma factor antagonist	-
MUA23_RS04140 (MUA23_04140)	856918..857394	+	477	WP_025904514.1	anti-sigma B factor RsbW	-
MUA23_RS04145 (MUA23_04145)	857369..858139	+	771	WP_037589755.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13653.83 Da
• Isoelectric Point: 9.9193

>T240597 WP_048540867.1 NZ_CP094816:855013-855384 [Mammaliicoccus sciuri]
MRRGDVYLADLSPVTGSEQGGTRPVVIIQNDTGNRYSPTVIVAAITGKINKAKIPTHVEIEAAKYKLDRDSVILLEQIRT
IDKKRLKEKLTYLSDAKMKEVDIAIAISLNLTLQQKFDTLGNT

Nucleotide

• Length: 372 bp

>T240597 NZ_LT598663:204104-204370 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6120.58 Da
• Isoelectric Point: 4.0121

>AT240597 WP_048540868.1 NZ_CP094816:854845-855012 [Mammaliicoccus sciuri]
MTSLTQNRTQSLEQTLKEGYRSMADLNLSLASEAYYSECEACDSNESHLASQQGE

Nucleotide

• Length: 168 bp

>AT240597 NZ_LT598663:203841-204104 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A418JBZ2