Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2838938..2839593 Replicon chromosome
Accession NC_012943
Organism Mycobacterium tuberculosis KZN 1435

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA6
Locus tag TBMG_RS13435 Protein ID WP_003898861.1
Coordinates 2839192..2839593 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TIK2
Locus tag TBMG_RS13430 Protein ID WP_003407272.1
Coordinates 2838938..2839195 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBMG_RS13410 2834125..2836092 - 1968 WP_003407285.1 primosomal protein N' -
TBMG_RS13415 2836173..2836910 - 738 WP_031647000.1 lysoplasmalogenase -
TBMG_RS13420 2836909..2837871 + 963 WP_003407279.1 alpha/beta hydrolase -
TBMG_RS13425 2837896..2838855 + 960 WP_003407276.1 alpha/beta hydrolase -
TBMG_RS13430 2838938..2839195 + 258 WP_003407272.1 ribbon-helix-helix domain-containing protein Antitoxin
TBMG_RS13435 2839192..2839593 + 402 WP_003898861.1 type II toxin-antitoxin system VapC family toxin Toxin
TBMG_RS20660 2839848..2841577 + 1730 Protein_2640 PE family protein -
TBMG_RS13450 2841623..2842657 - 1035 WP_003407264.1 AraC family transcriptional regulator -
TBMG_RS13455 2842735..2844120 + 1386 WP_003407260.1 cytochrome P450 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14952.43 Da        Isoelectric Point: 11.4656

>T24058 WP_003898861.1 NC_012943:2839192-2839593 [Mycobacterium tuberculosis KZN 1435]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQDLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T24058 NC_012943:2839192-2839593 [Mycobacterium tuberculosis KZN 1435]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT24058 WP_003407272.1 NC_012943:2838938-2839195 [Mycobacterium tuberculosis KZN 1435]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT24058 NC_012943:2838938-2839195 [Mycobacterium tuberculosis KZN 1435]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045ISB0

Antitoxin

Source ID Structure
AlphaFold DB G0TIK2

References