Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2648082..2648695 Replicon chromosome
Accession NC_012943
Organism Mycobacterium tuberculosis KZN 1435

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag TBMG_RS12575 Protein ID WP_003407786.1
Coordinates 2648082..2648486 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag TBMG_RS12580 Protein ID WP_009935474.1
Coordinates 2648492..2648695 (-) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBMG_RS12565 2644033..2646330 + 2298 WP_003407790.1 malto-oligosyltrehalose synthase -
TBMG_RS12570 2646323..2648065 + 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
TBMG_RS12575 2648082..2648486 - 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
TBMG_RS12580 2648492..2648695 - 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
TBMG_RS12585 2648748..2650037 - 1290 WP_003407781.1 threonine ammonia-lyase -
TBMG_RS12590 2650072..2650518 - 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
TBMG_RS12595 2650528..2651676 - 1149 Protein_2471 MMPL family transporter -
TBMG_RS12600 2651860..2652468 - 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
TBMG_RS12605 2652536..2652913 - 378 WP_003407771.1 fumarate reductase subunit FrdD -
TBMG_RS12610 2652910..2653290 - 381 WP_003407768.1 fumarate reductase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T24056 WP_003407786.1 NC_012943:c2648486-2648082 [Mycobacterium tuberculosis KZN 1435]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T24056 NC_012943:c2648486-2648082 [Mycobacterium tuberculosis KZN 1435]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT24056 WP_009935474.1 NC_012943:c2648695-2648492 [Mycobacterium tuberculosis KZN 1435]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT24056 NC_012943:c2648695-2648492 [Mycobacterium tuberculosis KZN 1435]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References