Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2475060..2475673 Replicon chromosome
Accession NC_012943
Organism Mycobacterium tuberculosis KZN 1435

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag TBMG_RS11835 Protein ID WP_003408465.1
Coordinates 2475284..2475673 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag TBMG_RS11830 Protein ID WP_003408469.1
Coordinates 2475060..2475287 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBMG_RS11810 2470952..2471662 + 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -
TBMG_RS11815 2471652..2472071 + 420 WP_003408483.1 hypothetical protein -
TBMG_RS11820 2472114..2473361 - 1248 WP_003408476.1 serine hydrolase -
TBMG_RS11825 2473358..2474842 - 1485 WP_009937637.1 biotin carboxylase -
TBMG_RS11830 2475060..2475287 + 228 WP_003408469.1 antitoxin Antitoxin
TBMG_RS11835 2475284..2475673 + 390 WP_003408465.1 PIN domain-containing protein Toxin
TBMG_RS11850 2476283..2477062 - 780 WP_003408460.1 IclR family transcriptional regulator -
TBMG_RS11855 2477076..2477894 - 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
TBMG_RS11860 2477947..2478296 - 350 Protein_2324 cupin domain-containing protein -
TBMG_RS11865 2478296..2479126 - 831 WP_003906661.1 cyclase family protein -
TBMG_RS11870 2479129..2480043 - 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T24055 WP_003408465.1 NC_012943:2475284-2475673 [Mycobacterium tuberculosis KZN 1435]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T24055 NC_012943:2475284-2475673 [Mycobacterium tuberculosis KZN 1435]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT24055 WP_003408469.1 NC_012943:2475060-2475287 [Mycobacterium tuberculosis KZN 1435]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT24055 NC_012943:2475060-2475287 [Mycobacterium tuberculosis KZN 1435]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References