Detailed information of TA system TA024054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2454481..2454941
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TKL9
• Locus tag: TBMG_RS11725
• Protein ID: WP_003898996.1
• Coordinates: 2454481..2454729 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: TBMG_RS11730
• Protein ID: WP_009938412.1
• Coordinates: 2454729..2454941 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS11705	2450655..2451266	+	612	WP_003898999.1	isopentenyl-diphosphate Delta-isomerase	-
TBMG_RS11710	2451256..2451657	+	402	WP_003408538.1	hypothetical protein	-
TBMG_RS11715	2451942..2453642	-	1701	WP_003898998.1	serine/threonine protein kinase PknE	-
TBMG_RS11720	2453736..2454473	-	738	WP_003898997.1	hypothetical protein	-
TBMG_RS11725	2454481..2454729	-	249	WP_003898996.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBMG_RS11730	2454729..2454941	-	213	WP_009938412.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBMG_RS11735	2455009..2456691	+	1683	WP_003898994.1	SulP family inorganic anion transporter	-
TBMG_RS11740	2456705..2456989	-	285	WP_003408522.1	DUF1876 domain-containing protein	-
TBMG_RS11745	2457276..2458463	+	1188	WP_003898992.1	nitrate transporter NarK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin VapB-like (2-69)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 8884.14 Da
• Isoelectric Point: 4.1074

>T24054 WP_003898996.1 NC_012943:c2454729-2454481 [Mycobacterium tuberculosis KZN 1435]
MVIDTSALVAMLNDEPEAQRFEIAVAADHVWLMSTASYPEMATVIETRFGEPGGREPKVSGQPLLYKGDDFACIDIRAVL
AG

Nucleotide

• Length: 249 bp

>T24054 NC_012943:c2454729-2454481 [Mycobacterium tuberculosis KZN 1435]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7665.84 Da
• Isoelectric Point: 7.9917

>AT24054 WP_009938412.1 NC_012943:c2454941-2454729 [Mycobacterium tuberculosis KZN 1435]
MELAARMGETLTQAVVVAVREQLARRTGRTRSISLREELAAIGRRCEALPVLDTRAADTILGYDERGLPA

Nucleotide

• Length: 213 bp

>AT24054 NC_012943:c2454941-2454729 [Mycobacterium tuberculosis KZN 1435]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGAGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• AlphaFold DB: G0TKL9