Detailed information of TA system TA024053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2227726..2228429
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: TBMG_RS10720
• Protein ID: WP_003409778.1
• Coordinates: 2228100..2228429 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: TBMG_RS21295
• Protein ID: WP_003409780.1
• Coordinates: 2227726..2228103 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS10675	2222884..2223075	+	192	WP_003409876.1	hypothetical protein	-
TBMG_RS10680	2223096..2223998	+	903	WP_003899097.1	hypothetical protein	-
TBMG_RS10685	2224009..2224359	+	351	WP_003899096.1	hypothetical protein	-
TBMG_RS10690	2224473..2224655	+	183	WP_003409870.1	hypothetical protein	-
TBMG_RS10695	2224648..2225049	-	402	WP_003409869.1	hypothetical protein	-
TBMG_RS10700	2225113..2225565	+	453	WP_003899095.1	lipoprotein	-
TBMG_RS10705	2225720..2227084	-	1365	WP_003903691.1	HNH endonuclease	-
TBMG_RS10710	2227139..2227729	+	591	WP_003409784.1	SEC-C domain-containing protein	-
TBMG_RS21295	2227726..2228103	+	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
TBMG_RS10720	2228100..2228429	+	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TBMG_RS10725	2228639..2229409	-	771	WP_003409775.1	SDR family oxidoreductase	-
TBMG_RS10730	2229406..2230467	-	1062	WP_009939004.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
TBMG_RS10735	2230464..2230979	-	516	WP_003409718.1	flavin reductase family protein	-
TBMG_RS10740	2230976..2232046	-	1071	WP_003899091.1	epoxide hydrolase EphB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T24053 WP_003409778.1 NC_012943:2228100-2228429 [Mycobacterium tuberculosis KZN 1435]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T24053 NC_012943:2228100-2228429 [Mycobacterium tuberculosis KZN 1435]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT24053 WP_003409780.1 NC_012943:2227726-2228103 [Mycobacterium tuberculosis KZN 1435]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT24053 NC_012943:2227726-2228103 [Mycobacterium tuberculosis KZN 1435]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8