Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2114418..2114947 Replicon chromosome
Accession NZ_CP094797
Organism Staphylococcus aureus strain IVB6163

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MUA16_RS10505 Protein ID WP_000621176.1
Coordinates 2114418..2114780 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MUA16_RS10510 Protein ID WP_000948331.1
Coordinates 2114777..2114947 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA16_RS10485 (2111396) 2111396..2112166 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MUA16_RS10490 (2112141) 2112141..2112620 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MUA16_RS10495 (2112622) 2112622..2112948 - 327 WP_001052491.1 anti-sigma factor antagonist -
MUA16_RS10500 (2113067) 2113067..2114068 - 1002 WP_262515884.1 PP2C family protein-serine/threonine phosphatase -
MUA16_RS10505 (2114418) 2114418..2114780 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUA16_RS10510 (2114777) 2114777..2114947 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MUA16_RS10515 (2115032) 2115032..2116180 - 1149 WP_262515887.1 alanine racemase -
MUA16_RS10520 (2116246) 2116246..2116605 - 360 WP_000581197.1 holo-ACP synthase -
MUA16_RS10525 (2116609) 2116609..2117100 - 492 WP_001286801.1 PH domain-containing protein -
MUA16_RS10530 (2117093) 2117093..2118670 - 1578 WP_262515888.1 PH domain-containing protein -
MUA16_RS10535 (2118663) 2118663..2119142 - 480 WP_001287082.1 hypothetical protein -
MUA16_RS10540 (2119351) 2119351..2119911 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T240493 WP_000621176.1 NZ_CP094797:c2114780-2114418 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T240493 NZ_LT556084:4384230-4384595 [Citrobacter amalonaticus]
ATGCAAATTTCAACTGCACCGACCACGGTGCCTGTTTCGTCACGCCTGACGCCTATCGAGGTCTGGCAGATACTACTCAA
ACATCTTCTGGCACAACACTATGGTCTGACCCTTAATGACACTCCGTTCAGCGATGAAAGCACTATCCGTCAACATATCA
ATGCTGGTATCTCACTCAGCAATGCAGTGAATTTTATCGTCGAAAAATATGAGCTGGTGCGTACTGACCGTAGTAGATTT
ACGATTATGGCGCAGTCTCCCTTCATCAGCAGTATCGAAATTCTTCGTGCCCGCCGGGCCACTGGATTGATGAAACGTCA
GGGTTATAAATCCATCAGTAATATCACTACTGGTCACCTTCTCTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT240493 WP_000948331.1 NZ_CP094797:c2114947-2114777 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT240493 NZ_LT556084:4383856-4384194 [Citrobacter amalonaticus]
ATGACTAAAGAACCTGTTCAGGAACACGTTTGGGGCTTACACAGTCAAACCGTTCCATCATTCAGCGCCCGGTTAGTTCA
GGAAGGTCGACGGCTGCATTTTCTGGCCGACCGTGCCACGCTTCTCGGCACATTCAGCCCAGAACAATCCGCAACGCTGG
ATGCGATCTTCCCAGCATTAATCCGGGGTATGGAAACCGCTCTGCTTAGCGGCAAACTCGACCCACGTAAGGCTAAACAA
TACATCTATATCCGCAACGGATTCGCCTGCGAGTCAGATACGTTGGGGAGTTGCGGTTACGTTTACATCGTAATTTATCC
TCAATCAGAAACCAGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References