Detailed information of TA system TA024047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2164401..2165027
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: TBMG_RS10335
• Protein ID: WP_003410075.1
• Coordinates: 2164401..2164799 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: TBMG_RS10340
• Protein ID: WP_003911750.1
• Coordinates: 2164800..2165027 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS10310	2160000..2161256	+	1257	WP_003410095.1	HNH endonuclease	-
TBMG_RS10315	2161384..2161974	-	591	WP_003899131.1	IS110 family transposase	-
TBMG_RS10320	2161928..2162575	-	648	WP_071854215.1	transposase	-
TBMG_RS21270	2162636..2162815	+	180	Protein_2018	hypothetical protein	-
TBMG_RS10325	2163252..2163686	-	435	WP_003914358.1	DUF1398 domain-containing protein	-
TBMG_RS10330	2163787..2164218	+	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
TBMG_RS10335	2164401..2164799	-	399	WP_003410075.1	PIN domain nuclease	Toxin
TBMG_RS10340	2164800..2165027	-	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBMG_RS10345	2165286..2166455	+	1170	WP_003899126.1	ATP-binding protein	-
TBMG_RS10350	2166644..2166988	+	345	WP_003410065.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T24047 WP_003410075.1 NC_012943:c2164799-2164401 [Mycobacterium tuberculosis KZN 1435]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T24047 NC_012943:c2164799-2164401 [Mycobacterium tuberculosis KZN 1435]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT24047 WP_003911750.1 NC_012943:c2165027-2164800 [Mycobacterium tuberculosis KZN 1435]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT24047 NC_012943:c2165027-2164800 [Mycobacterium tuberculosis KZN 1435]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8