Detailed information of TA system TA240468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2117387..2117916
• Replicon: chromosome
• Accession: NZ_CP094793
• Organism: Staphylococcus aureus strain IVB6165

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA93_RS10410
• Protein ID: WP_000621175.1
• Coordinates: 2117387..2117749 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUA93_RS10415
• Protein ID: WP_000948331.1
• Coordinates: 2117746..2117916 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA93_RS10390 (MUA93_10380)	2114365..2115135	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
MUA93_RS10395 (MUA93_10385)	2115110..2115589	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
MUA93_RS10400 (MUA93_10390)	2115591..2115917	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MUA93_RS10405 (MUA93_10395)	2116036..2117037	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MUA93_RS10410 (MUA93_10400)	2117387..2117749	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA93_RS10415 (MUA93_10405)	2117746..2117916	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA93_RS10420 (MUA93_10410)	2118001..2119149	-	1149	WP_001281160.1	alanine racemase	-
MUA93_RS10425 (MUA93_10415)	2119215..2119574	-	360	WP_000581198.1	holo-ACP synthase	-
MUA93_RS10430 (MUA93_10420)	2119578..2120069	-	492	WP_001205919.1	PH domain-containing protein	-
MUA93_RS10435 (MUA93_10425)	2120056..2121639	-	1584	WP_252550166.1	PH domain-containing protein	-
MUA93_RS10440 (MUA93_10430)	2121632..2122111	-	480	WP_001287083.1	hypothetical protein	-
MUA93_RS10445 (MUA93_10435)	2122320..2122880	-	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240468 WP_000621175.1 NZ_CP094793:c2117749-2117387 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240468 NZ_LT220504:2653240-2653623 [Lacticaseibacillus rhamnosus]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACCCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240468 WP_000948331.1 NZ_CP094793:c2117916-2117746 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240468 NZ_LT220504:2652972-2653220 [Lacticaseibacillus rhamnosus]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAATTGATTT
CTGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1