Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2234790..2235319 Replicon chromosome
Accession NZ_CP094785
Organism Staphylococcus aureus strain IVB6169

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MUA79_RS11300 Protein ID WP_000621176.1
Coordinates 2234790..2235152 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MUA79_RS11305 Protein ID WP_000948331.1
Coordinates 2235149..2235319 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA79_RS11280 (MUA79_11265) 2231768..2232538 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MUA79_RS11285 (MUA79_11270) 2232513..2232992 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MUA79_RS11290 (MUA79_11275) 2232994..2233320 - 327 WP_001052491.1 anti-sigma factor antagonist -
MUA79_RS11295 (MUA79_11280) 2233439..2234440 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
MUA79_RS11300 (MUA79_11285) 2234790..2235152 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUA79_RS11305 (MUA79_11290) 2235149..2235319 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MUA79_RS11310 (MUA79_11295) 2235404..2236552 - 1149 WP_001281140.1 alanine racemase -
MUA79_RS11315 (MUA79_11300) 2236618..2236977 - 360 WP_000581197.1 holo-ACP synthase -
MUA79_RS11320 (MUA79_11305) 2236981..2237472 - 492 WP_001286801.1 PH domain-containing protein -
MUA79_RS11325 (MUA79_11310) 2237465..2239042 - 1578 Protein_2192 PH domain-containing protein -
MUA79_RS11330 (MUA79_11315) 2239035..2239514 - 480 WP_001287082.1 hypothetical protein -
MUA79_RS11335 (MUA79_11320) 2239723..2240283 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T240407 WP_000621176.1 NZ_CP094785:c2235152-2234790 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T240407 NZ_LS999206:c298745-298458 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTGAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT240407 WP_000948331.1 NZ_CP094785:c2235319-2235149 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT240407 NZ_LS999206:c299054-298752 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References