Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1543450..1544087 Replicon chromosome
Accession NC_012943
Organism Mycobacterium tuberculosis KZN 1435

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag TBMG_RS07380 Protein ID WP_003413180.1
Coordinates 1543692..1544087 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag TBMG_RS07375 Protein ID WP_003413183.1
Coordinates 1543450..1543695 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBMG_RS07355 1540044..1540556 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
TBMG_RS07360 1540549..1541802 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
TBMG_RS07365 1541799..1542608 + 810 WP_003413193.1 shikimate dehydrogenase -
TBMG_RS07370 1542620..1543039 + 420 WP_003413190.1 A24 family peptidase -
TBMG_RS07375 1543450..1543695 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
TBMG_RS07380 1543692..1544087 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
TBMG_RS07385 1544187..1544561 + 375 WP_003413177.1 hypothetical protein -
TBMG_RS07390 1544577..1544954 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
TBMG_RS07395 1544951..1545208 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
TBMG_RS07400 1545247..1545660 - 414 WP_003413164.1 PIN domain nuclease -
TBMG_RS07405 1545753..1546031 - 279 WP_012775752.1 type II toxin-antitoxin system VapB family antitoxin -
TBMG_RS07410 1546028..1546690 - 663 WP_003900848.1 hypothetical protein -
TBMG_RS07415 1546687..1547346 - 660 WP_003900846.1 LppA family lipoprotein -
TBMG_RS07420 1547473..1548684 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T24038 WP_003413180.1 NC_012943:1543692-1544087 [Mycobacterium tuberculosis KZN 1435]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T24038 NC_012943:1543692-1544087 [Mycobacterium tuberculosis KZN 1435]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT24038 WP_003413183.1 NC_012943:1543450-1543695 [Mycobacterium tuberculosis KZN 1435]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT24038 NC_012943:1543450-1543695 [Mycobacterium tuberculosis KZN 1435]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References