Detailed information of TA system TA240372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2104942..2105471
• Replicon: chromosome
• Accession: NZ_CP094782
• Organism: Staphylococcus aureus strain IVB6172

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA59_RS10565
• Protein ID: WP_000621175.1
• Coordinates: 2104942..2105304 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUA59_RS10570
• Protein ID: WP_000948331.1
• Coordinates: 2105301..2105471 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA59_RS10545 (MUA59_10515)	2101920..2102690	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
MUA59_RS10550 (MUA59_10520)	2102665..2103144	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
MUA59_RS10555 (MUA59_10525)	2103146..2103472	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MUA59_RS10560 (MUA59_10530)	2103591..2104592	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MUA59_RS10565 (MUA59_10535)	2104942..2105304	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA59_RS10570 (MUA59_10540)	2105301..2105471	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA59_RS10575 (MUA59_10545)	2105556..2106704	-	1149	WP_001281143.1	alanine racemase	-
MUA59_RS10580 (MUA59_10550)	2106770..2107129	-	360	WP_000581197.1	holo-ACP synthase	-
MUA59_RS10585 (MUA59_10555)	2107133..2107624	-	492	WP_262538389.1	PH domain-containing protein	-
MUA59_RS10590 (MUA59_10560)	2107617..2109194	-	1578	WP_262613786.1	PH domain-containing protein	-
MUA59_RS10595 (MUA59_10565)	2109187..2109666	-	480	WP_001287083.1	hypothetical protein	-
MUA59_RS10600 (MUA59_10570)	2109875..2110435	-	561	WP_025788990.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240372 WP_000621175.1 NZ_CP094782:c2105304-2104942 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240372 NZ_LS998783:2588624-2588875 [Pseudomonas aeruginosa]
ATGCGCACTATCAGCTATACCGAAGCCCGGCAGAACCTGTCGGCCACCATGGTGCAGACCGTCGAGGATCGCGTGCCTAC
CCTCATTACCCGCCAGAACGGCGAGGCGTGCGTGCTGATGTCGCTGGAGGAGTACAACGCGCTGGAGGAAACGGCTTACC
TGCTGCGCTCGCCGGCCAACGCGCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCGTGGCCAGGGTGTCGAGCGGGAT
ATCGCCGAGTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240372 WP_000948331.1 NZ_CP094782:c2105471-2105301 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240372 NZ_LS998783:2588872-2589126 [Pseudomonas aeruginosa]
GTGAAGCTGATCTGGTCTGAGCAAGCCTGGGACGATTATCTGTACTGGCAAGAGGCGGATAAGCGTATGGTCAAGAAGCT
CAACGAGCTGATCAAGGAGACCCGCCGCACCCCTTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGGCGGATCACCGCCGAGCACCGGTTTGTGTATTCGGTGACGGACGAGGCGCTGCTGATTGCCGCCTGC
CGTTATCACTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1