Detailed information of TA system TA240360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2117386..2117915
• Replicon: chromosome
• Accession: NZ_CP094780
• Organism: Staphylococcus aureus strain IVB6173

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA18_RS10390
• Protein ID: WP_000621175.1
• Coordinates: 2117386..2117748 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUA18_RS10395
• Protein ID: WP_000948331.1
• Coordinates: 2117745..2117915 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA18_RS10370 (MUA18_10365)	2114364..2115134	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
MUA18_RS10375 (MUA18_10370)	2115109..2115588	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
MUA18_RS10380 (MUA18_10375)	2115590..2115916	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MUA18_RS10385 (MUA18_10380)	2116035..2117036	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MUA18_RS10390 (MUA18_10385)	2117386..2117748	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA18_RS10395 (MUA18_10390)	2117745..2117915	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA18_RS10400 (MUA18_10395)	2118000..2119148	-	1149	WP_001281160.1	alanine racemase	-
MUA18_RS10405 (MUA18_10400)	2119214..2119573	-	360	WP_000581198.1	holo-ACP synthase	-
MUA18_RS10410 (MUA18_10405)	2119577..2120068	-	492	WP_001205919.1	PH domain-containing protein	-
MUA18_RS10415 (MUA18_10410)	2120055..2121638	-	1584	WP_252550166.1	PH domain-containing protein	-
MUA18_RS10420 (MUA18_10415)	2121631..2122110	-	480	WP_001287083.1	hypothetical protein	-
MUA18_RS10425 (MUA18_10420)	2122319..2122879	-	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240360 WP_000621175.1 NZ_CP094780:c2117748-2117386 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240360 NZ_LS997868:574316-574579 [Vibrio cholerae]
ATGAGTAGTCGTTTACTGTCATGGACAGATGAGTCCTGGGATGACTACTTGTACTGGCAAACACAAGACAAAAAAACACT
CAAGCGGATCAATAAAATCATCAATGATGTTAAGCGTTCACCGTTTGAAGGTATTGGTAAACCAGAGCCTCTAAAAGAAA
ATCTATCTGGTTTTTGGTCTCGACGGATCGATGACACAAATAGACTGGTTTATGCTGTTGATGATAATGCAATAACGATT
ATTTCGTGTCGCTACCATTATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240360 WP_000948331.1 NZ_CP094780:c2117915-2117745 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240360 NZ_LS997868:574069-574323 [Vibrio cholerae]
ATGAGAATCGTTTCTTTTACAGAAGCTAGGAATAGTTTAAAAGCAGTCTTGGATGCTGTTGTGAATGATGCGGATACAAC
CGTCATCACTCGACGTGATTCAGAAGATGCCGTGGTTATGTCGCTGGATTATTACAATAGTCTAATGGAAACCGTACATT
TGCTTCGCTCTCCAGCGAACGCTGAACATCTAAACCGTTCCATTGCCCAGTTCAAAGCGGGAAAAGTTACCCAAAGAGAT
CTGATTGATGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1