Detailed information of TA system TA024035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1344167..1344846
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: TBMG_RS06310
• Protein ID: WP_003414059.1
• Coordinates: 1344430..1344846 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: TBMG_RS06305
• Protein ID: WP_003414061.1
• Coordinates: 1344167..1344433 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS06270	1339643..1340380	-	738	WP_003414079.1	dienelactone hydrolase family protein	-
TBMG_RS06275	1340536..1341336	+	801	WP_009939104.1	thymidylate synthase	-
TBMG_RS06280	1341407..1341886	+	480	WP_003414073.1	dihydrofolate reductase	-
TBMG_RS06285	1341960..1342379	+	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
TBMG_RS06290	1342376..1343470	+	1095	WP_003414068.1	restriction endonuclease subunit S	-
TBMG_RS06295	1343480..1343749	+	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
TBMG_RS06300	1343746..1344141	+	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
TBMG_RS06305	1344167..1344433	+	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBMG_RS06310	1344430..1344846	+	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
TBMG_RS06315	1344933..1346555	+	1623	WP_003906897.1	type I restriction-modification system subunit M	-
TBMG_RS06320	1346552..1346827	+	276	WP_003414055.1	type I restriction endonuclease subunit S	-
TBMG_RS06325	1347071..1347823	+	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
TBMG_RS06330	1347892..1348794	+	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T24035 WP_003414059.1 NC_012943:1344430-1344846 [Mycobacterium tuberculosis KZN 1435]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T24035 NC_012943:1344430-1344846 [Mycobacterium tuberculosis KZN 1435]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT24035 WP_003414061.1 NC_012943:1344167-1344433 [Mycobacterium tuberculosis KZN 1435]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT24035 NC_012943:1344167-1344433 [Mycobacterium tuberculosis KZN 1435]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64