Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2176345..2176874 Replicon chromosome
Accession NZ_CP094778
Organism Staphylococcus aureus strain IVB6185

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MUA10_RS10815 Protein ID WP_000621176.1
Coordinates 2176345..2176707 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MUA10_RS10820 Protein ID WP_000948331.1
Coordinates 2176704..2176874 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA10_RS10795 (MUA10_10790) 2173323..2174093 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MUA10_RS10800 (MUA10_10795) 2174068..2174547 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MUA10_RS10805 (MUA10_10800) 2174549..2174875 - 327 WP_001052491.1 anti-sigma factor antagonist -
MUA10_RS10810 (MUA10_10805) 2174994..2175995 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
MUA10_RS10815 (MUA10_10810) 2176345..2176707 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUA10_RS10820 (MUA10_10815) 2176704..2176874 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MUA10_RS10825 (MUA10_10820) 2176959..2178107 - 1149 WP_001281140.1 alanine racemase -
MUA10_RS10830 (MUA10_10825) 2178173..2178532 - 360 WP_000581197.1 holo-ACP synthase -
MUA10_RS10835 (MUA10_10830) 2178536..2179027 - 492 WP_001286801.1 PH domain-containing protein -
MUA10_RS10840 (MUA10_10835) 2179020..2180597 - 1578 WP_001294646.1 PH domain-containing protein -
MUA10_RS10845 (MUA10_10840) 2180590..2181069 - 480 WP_001287082.1 hypothetical protein -
MUA10_RS10850 (MUA10_10845) 2181278..2181838 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T240347 WP_000621176.1 NZ_CP094778:c2176707-2176345 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T240347 NZ_LS992193:c76841-76683 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT240347 WP_000948331.1 NZ_CP094778:c2176874-2176704 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT240347 NZ_LS992193:c76916-76885 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References