Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 2020459..2021235 Replicon chromosome
Accession NZ_CP094778
Organism Staphylococcus aureus strain IVB6185

Toxin (Protein)


Gene name tsbT Uniprot ID X5E2E6
Locus tag MUA10_RS09930 Protein ID WP_000031108.1
Coordinates 2020459..2020611 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID A0A0D1IYN8
Locus tag MUA10_RS09935 Protein ID WP_001251219.1
Coordinates 2020636..2021235 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA10_RS09915 (MUA10_09910) 2016604..2017425 + 822 WP_000669380.1 RluA family pseudouridine synthase -
MUA10_RS09920 (MUA10_09915) 2017881..2019266 - 1386 WP_000116227.1 class II fumarate hydratase -
MUA10_RS09925 (MUA10_09920) 2019462..2019857 - 396 WP_045179323.1 hypothetical protein -
MUA10_RS09930 (MUA10_09925) 2020459..2020611 - 153 WP_000031108.1 SAS053 family protein Toxin
MUA10_RS09935 (MUA10_09930) 2020636..2021235 - 600 WP_001251219.1 glucosamine-6-phosphate isomerase Antitoxin
MUA10_RS09940 (MUA10_09935) 2021394..2021864 - 471 WP_000181394.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
MUA10_RS09945 (MUA10_09940) 2021869..2022996 - 1128 WP_000379980.1 tRNA epoxyqueuosine(34) reductase QueG -
MUA10_RS09950 (MUA10_09945) 2023147..2023869 - 723 WP_000590809.1 amino acid ABC transporter ATP-binding protein -
MUA10_RS09955 (MUA10_09950) 2023862..2025319 - 1458 WP_000649898.1 ABC transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5936.28 Da        Isoelectric Point: 3.8962

>T240346 WP_000031108.1 NZ_CP094778:c2020611-2020459 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T240346 NZ_LS992193:c34282-34076 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22357.50 Da        Isoelectric Point: 5.1445

>AT240346 WP_001251219.1 NZ_CP094778:c2021235-2020636 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNIEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT240346 NZ_LS992193:34269-34330 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X5E2E6

Antitoxin

Source ID Structure
AlphaFold DB A0A0D1IYN8

References