Detailed information of TA system TA024033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1302920..1303490
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: TBMG_RS06075
• Protein ID: WP_003414166.1
• Coordinates: 1303134..1303490 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: TBMG_RS06070
• Protein ID: WP_003901465.1
• Coordinates: 1302920..1303150 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS06030	1297938..1298249	-	312	WP_003414190.1	hypothetical protein	-
TBMG_RS06035	1298354..1298611	-	258	WP_003899489.1	hypothetical protein	-
TBMG_RS06040	1298845..1300134	-	1290	Protein_1188	transposase family protein	-
TBMG_RS06045	1300198..1300389	-	192	WP_003414184.1	hypothetical protein	-
TBMG_RS06050	1300386..1300790	-	405	WP_009938577.1	hypothetical protein	-
TBMG_RS22100	1300938..1301192	+	255	WP_003917684.1	hypothetical protein	-
TBMG_RS22105	1301368..1301643	-	276	WP_003911993.1	DUF1778 domain-containing protein	-
TBMG_RS06065	1301834..1302877	+	1044	WP_003414172.1	DUF2293 domain-containing protein	-
TBMG_RS06070	1302920..1303150	+	231	WP_003901465.1	antitoxin MazE	Antitoxin
TBMG_RS06075	1303134..1303490	+	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
TBMG_RS06080	1303592..1305241	-	1650	WP_003899485.1	CocE/NonD family hydrolase	-
TBMG_RS06085	1305260..1305847	-	588	WP_003914429.1	DUF3558 family protein	-
TBMG_RS06090	1306020..1306346	+	327	WP_003414157.1	hypothetical protein	-
TBMG_RS06095	1306350..1308038	+	1689	WP_003414155.1	alpha/beta hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1292281..1331380	39099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T24033 WP_003414166.1 NC_012943:1303134-1303490 [Mycobacterium tuberculosis KZN 1435]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T24033 NC_012943:1303134-1303490 [Mycobacterium tuberculosis KZN 1435]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT24033 WP_003901465.1 NC_012943:1302920-1303150 [Mycobacterium tuberculosis KZN 1435]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT24033 NC_012943:1302920-1303150 [Mycobacterium tuberculosis KZN 1435]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3