Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 1276603..1277207 Replicon chromosome
Accession NC_012943
Organism Mycobacterium tuberculosis KZN 1435

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag TBMG_RS05925 Protein ID WP_003414492.1
Coordinates 1276815..1277207 (+) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag TBMG_RS05920 Protein ID WP_003414495.1
Coordinates 1276603..1276818 (+) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBMG_RS05895 1271605..1272516 + 912 WP_003414505.1 sugar ABC transporter permease -
TBMG_RS05900 1272513..1273340 + 828 WP_003414504.1 carbohydrate ABC transporter permease -
TBMG_RS05905 1273343..1274653 + 1311 WP_003414501.1 ABC transporter substrate-binding protein -
TBMG_RS05910 1274646..1275728 + 1083 WP_003414499.1 ABC transporter ATP-binding protein -
TBMG_RS05915 1275807..1276556 - 750 WP_003414497.1 enoyl-CoA hydratase -
TBMG_RS05920 1276603..1276818 + 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
TBMG_RS05925 1276815..1277207 + 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
TBMG_RS05930 1277228..1277497 + 270 WP_003414489.1 DUF2277 family protein -
TBMG_RS05935 1277494..1278039 + 546 WP_009939112.1 DUF1802 family protein -
TBMG_RS05940 1278344..1279231 + 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
TBMG_RS05945 1279234..1280118 + 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
TBMG_RS05950 1280390..1280935 + 546 WP_003913758.1 DUF1802 family protein -
TBMG_RS05955 1281269..1282057 + 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T24031 WP_003414492.1 NC_012943:1276815-1277207 [Mycobacterium tuberculosis KZN 1435]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T24031 NC_012943:1276815-1277207 [Mycobacterium tuberculosis KZN 1435]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT24031 WP_003414495.1 NC_012943:1276603-1276818 [Mycobacterium tuberculosis KZN 1435]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT24031 NC_012943:1276603-1276818 [Mycobacterium tuberculosis KZN 1435]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References