Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2134109..2134638 Replicon chromosome
Accession NZ_CP094772
Organism Staphylococcus aureus strain IVB6196

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MUA50_RS10685 Protein ID WP_000621176.1
Coordinates 2134109..2134471 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MUA50_RS10690 Protein ID WP_000948331.1
Coordinates 2134468..2134638 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA50_RS10665 (2131087) 2131087..2131857 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MUA50_RS10670 (2131832) 2131832..2132311 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MUA50_RS10675 (2132313) 2132313..2132639 - 327 WP_001052491.1 anti-sigma factor antagonist -
MUA50_RS10680 (2132758) 2132758..2133759 - 1002 WP_262515884.1 PP2C family protein-serine/threonine phosphatase -
MUA50_RS10685 (2134109) 2134109..2134471 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUA50_RS10690 (2134468) 2134468..2134638 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MUA50_RS10695 (2134723) 2134723..2135871 - 1149 WP_262515887.1 alanine racemase -
MUA50_RS10700 (2135937) 2135937..2136296 - 360 WP_000581197.1 holo-ACP synthase -
MUA50_RS10705 (2136300) 2136300..2136791 - 492 WP_001286801.1 PH domain-containing protein -
MUA50_RS10710 (2136784) 2136784..2138361 - 1578 WP_262515888.1 PH domain-containing protein -
MUA50_RS10715 (2138354) 2138354..2138833 - 480 WP_001287082.1 hypothetical protein -
MUA50_RS10720 (2139042) 2139042..2139602 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T240300 WP_000621176.1 NZ_CP094772:c2134471-2134109 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T240300 NZ_LS992190:c891067-890690 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT240300 WP_000948331.1 NZ_CP094772:c2134638-2134468 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT240300 NZ_LS992190:c891524-891144 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACTCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References