Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2147730..2148244 Replicon chromosome
Accession NZ_CP094770
Organism Staphylococcus aureus strain IVB6201

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag MUA35_RS10755 Protein ID WP_000581792.1
Coordinates 2147730..2147939 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A0D1GBN9
Locus tag MUA35_RS10760 Protein ID WP_000138934.1
Coordinates 2147951..2148244 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA35_RS10735 (MUA35_10700) 2143238..2144596 - 1359 WP_262607637.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MUA35_RS10740 (MUA35_10705) 2144611..2145681 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
MUA35_RS10745 (MUA35_10710) 2145999..2147201 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
MUA35_RS10750 (MUA35_10715) 2147465..2147602 - 138 WP_000828354.1 Lmo0850 family protein -
MUA35_RS10755 (MUA35_10720) 2147730..2147939 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
MUA35_RS10760 (MUA35_10725) 2147951..2148244 - 294 WP_000138934.1 copper-sensing transcriptional repressor CsoR Antitoxin
MUA35_RS10765 (MUA35_10730) 2148410..2149894 + 1485 WP_000571549.1 cardiolipin synthase -
MUA35_RS10770 (MUA35_10735) 2149922..2150569 + 648 WP_046463185.1 HD domain-containing protein -
MUA35_RS10775 (MUA35_10740) 2151441..2152313 - 873 WP_000725802.1 membrane protein insertase YidC -
MUA35_RS10780 (MUA35_10745) 2152399..2153040 - 642 WP_000483160.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(3-95)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T240283 WP_000581792.1 NZ_CP094770:c2147939-2147730 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T240283 NZ_LS992186:c30589-30284 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11185.77 Da        Isoelectric Point: 6.2243

>AT240283 WP_000138934.1 NZ_CP094770:c2148244-2147951 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT240283 NZ_LS992186:c30809-30591 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB A0A0D1GBN9

References