Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2220222..2220751 Replicon chromosome
Accession NZ_CP094765
Organism Staphylococcus aureus strain IVB6215

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MUA45_RS11190 Protein ID WP_000621176.1
Coordinates 2220222..2220584 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MUA45_RS11195 Protein ID WP_000948331.1
Coordinates 2220581..2220751 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA45_RS11170 (MUA45_11165) 2217200..2217970 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MUA45_RS11175 (MUA45_11170) 2217945..2218424 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MUA45_RS11180 (MUA45_11175) 2218426..2218752 - 327 WP_001052491.1 anti-sigma factor antagonist -
MUA45_RS11185 (MUA45_11180) 2218871..2219872 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
MUA45_RS11190 (MUA45_11185) 2220222..2220584 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUA45_RS11195 (MUA45_11190) 2220581..2220751 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MUA45_RS11200 (MUA45_11195) 2220836..2221984 - 1149 WP_001281140.1 alanine racemase -
MUA45_RS11205 (MUA45_11200) 2222050..2222409 - 360 WP_000581197.1 holo-ACP synthase -
MUA45_RS11210 (MUA45_11205) 2222413..2222904 - 492 WP_001286801.1 PH domain-containing protein -
MUA45_RS11215 (MUA45_11210) 2222897..2224474 - 1578 WP_262633673.1 PH domain-containing protein -
MUA45_RS11220 (MUA45_11215) 2224467..2224946 - 480 WP_001287082.1 hypothetical protein -
MUA45_RS11225 (MUA45_11220) 2225155..2225715 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T240260 WP_000621176.1 NZ_CP094765:c2220584-2220222 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T240260 NZ_LS992185:473164-473337 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT240260 WP_000948331.1 NZ_CP094765:c2220751-2220581 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT240260 NZ_LS992185:472840-473136 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References