Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2160806..2161320 Replicon chromosome
Accession NZ_CP094763
Organism Staphylococcus aureus strain IVB6221

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag MUA83_RS10665 Protein ID WP_000581792.1
Coordinates 2160806..2161015 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A0D1GBN9
Locus tag MUA83_RS10670 Protein ID WP_000138934.1
Coordinates 2161027..2161320 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA83_RS10645 (MUA83_10640) 2156314..2157672 - 1359 WP_000611474.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
MUA83_RS10650 (MUA83_10645) 2157687..2158757 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
MUA83_RS10655 (MUA83_10650) 2159075..2160277 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
MUA83_RS10660 (MUA83_10655) 2160541..2160678 - 138 WP_000828354.1 Lmo0850 family protein -
MUA83_RS10665 (MUA83_10660) 2160806..2161015 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
MUA83_RS10670 (MUA83_10665) 2161027..2161320 - 294 WP_000138934.1 copper-sensing transcriptional repressor CsoR Antitoxin
MUA83_RS10675 (MUA83_10670) 2161486..2162970 + 1485 WP_262529058.1 cardiolipin synthase -
MUA83_RS10680 (MUA83_10675) 2162998..2163645 + 648 WP_001187631.1 HD domain-containing protein -
MUA83_RS10685 (MUA83_10680) 2164317..2165189 - 873 WP_000725802.1 membrane protein insertase YidC -
MUA83_RS10690 (MUA83_10685) 2165275..2165916 - 642 WP_000483148.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(3-95)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T240248 WP_000581792.1 NZ_CP094763:c2161015-2160806 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T240248 NZ_LS992183:2397945-2398121 [Citrobacter freundii]
GTGAAGCAAAGTGAGTTTAGACGCTGGCTTGAATCTCAGGGAGTTGCTGTTACAAACGGCTCAAATCATCTGAAACTGAG
ATATCAGGGAAGGCGTAGCGTTATGCCCAGACATCCAGGTGATGAAATAAAAGAAGCCTTGCGAAAAGCGATTATTAAGC
AGCTTGACTTACAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11185.77 Da        Isoelectric Point: 6.2243

>AT240248 WP_000138934.1 NZ_CP094763:c2161320-2161027 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT240248 NZ_LS992183:2398168-2398584 [Citrobacter freundii]
ATGCGTTATCCTGTTACTCTAACACCGGCAGTGGAAGGTGGGTTTGTGGTTTCTTTCCCGGATATTCCGGAAGCATTAAC
TCAGGGAAATACTCGCCATGACGCTTTGCAGGCCGCGCAGGCGGCATTGATTACCGCTTTTGAATTCTATTTCGACGACA
ACGAGGCGATTCCACTCCCTTCAGCGGTTAGCGCTGAAGATGACTACGTAGAAATACCGCTGAGCGTTGCGTCTAAGGTT
CTTCTGCTGAATGCCTTCCTTGAATCAAAAATCACCCAGCAGGAGCTGGCGAACAGGATCGGCAGACCTAAGCAGGAGAT
CACCCGTTTATTTGATTTAAAGCACACGACAAAAATTGATGCCGTGCAGATTGCTGCGCGCGCCTTAGGCAAAGAGCTGG
CGTTAACGATGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB A0A0D1GBN9

References