Detailed information of TA system TA240232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2055304..2055833
• Replicon: chromosome
• Accession: NZ_CP094760
• Organism: Staphylococcus aureus strain IVB6232

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA14_RS09990
• Protein ID: WP_000621175.1
• Coordinates: 2055304..2055666 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUA14_RS09995
• Protein ID: WP_000948331.1
• Coordinates: 2055663..2055833 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA14_RS09970 (MUA14_09960)	2052283..2053053	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
MUA14_RS09975 (MUA14_09965)	2053028..2053507	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
MUA14_RS09980 (MUA14_09970)	2053509..2053835	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MUA14_RS09985 (MUA14_09975)	2053954..2054955	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MUA14_RS09990 (MUA14_09980)	2055304..2055666	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA14_RS09995 (MUA14_09985)	2055663..2055833	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA14_RS10000 (MUA14_09990)	2055918..2057066	-	1149	WP_001281160.1	alanine racemase	-
MUA14_RS10005 (MUA14_09995)	2057132..2057491	-	360	WP_000581198.1	holo-ACP synthase	-
MUA14_RS10010 (MUA14_10000)	2057495..2057986	-	492	WP_001205919.1	PH domain-containing protein	-
MUA14_RS10015 (MUA14_10005)	2057973..2059556	-	1584	WP_252550166.1	PH domain-containing protein	-
MUA14_RS10020 (MUA14_10010)	2059549..2060028	-	480	WP_001287083.1	hypothetical protein	-
MUA14_RS10025 (MUA14_10015)	2060237..2060797	-	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240232 WP_000621175.1 NZ_CP094760:c2055666-2055304 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240232 NZ_LS992180:c4928125-4927814 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240232 WP_000948331.1 NZ_CP094760:c2055833-2055663 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240232 NZ_LS992180:c4927813-4927523 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1