Detailed information of TA system TA240230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 871928..872405
• Replicon: chromosome
• Accession: NZ_CP094760
• Organism: Staphylococcus aureus strain IVB6232

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: MUA14_RS04180
• Protein ID: WP_000052076.1
• Coordinates: 871928..872116 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: MUA14_RS04185
• Protein ID: WP_001059690.1
• Coordinates: 872145..872405 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA14_RS04150 (MUA14_04140)	867917..868438	+	522	WP_000824476.1	hypothetical protein	-
MUA14_RS04155 (MUA14_04145)	868779..869465	+	687	WP_252550202.1	thermonuclease family protein	-
MUA14_RS04160 (MUA14_04150)	869822..870022	+	201	WP_001059082.1	cold-shock protein	-
MUA14_RS04165 (MUA14_04155)	870519..870737	-	219	WP_000005883.1	hypothetical protein	-
MUA14_RS04170 (MUA14_04160)	870799..871083	-	285	WP_001004401.1	hypothetical protein	-
MUA14_RS04175 (MUA14_04165)	871171..871740	-	570	WP_001106648.1	lipoprotein N-acylation protein LnsA	-
MUA14_RS04180 (MUA14_04170)	871928..872116	-	189	WP_000052076.1	hypothetical protein	Toxin
MUA14_RS04185 (MUA14_04175)	872145..872405	-	261	WP_001059690.1	hypothetical protein	Antitoxin
MUA14_RS04190 (MUA14_04180)	872746..872949	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
MUA14_RS04195 (MUA14_04185)	872946..873182	-	237	WP_000368687.1	hypothetical protein	-
MUA14_RS04200 (MUA14_04190)	873407..873988	+	582	WP_001140696.1	histidine phosphatase family protein	-
MUA14_RS04205 (MUA14_04195)	874061..874678	-	618	WP_000255565.1	LysE/ArgO family amino acid transporter	-
MUA14_RS04210 (MUA14_04200)	874834..875328	-	495	WP_000569881.1	GNAT family N-acetyltransferase	-
MUA14_RS04215 (MUA14_04205)	875727..876149	-	423	WP_000974462.1	organic hydroperoxide resistance protein	-
MUA14_RS04220 (MUA14_04210)	876297..877013	+	717	WP_000150032.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T240230 WP_000052076.1 NZ_CP094760:c872116-871928 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T240230 NZ_LS992180:c4508865-4508515 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT240230 WP_001059690.1 NZ_CP094760:c872405-872145 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT240230 NZ_LS992180:c4509110-4508859 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56