Detailed information of TA system TA240224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2088559..2089088
• Replicon: chromosome
• Accession: NZ_CP094758
• Organism: Staphylococcus aureus strain IVB6239

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA67_RS10455
• Protein ID: WP_000621175.1
• Coordinates: 2088559..2088921 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUA67_RS10460
• Protein ID: WP_000948331.1
• Coordinates: 2088918..2089088 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA67_RS10435 (2085538)	2085538..2086308	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
MUA67_RS10440 (2086283)	2086283..2086762	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
MUA67_RS10445 (2086764)	2086764..2087090	-	327	WP_262610201.1	anti-sigma factor antagonist	-
MUA67_RS10450 (2087209)	2087209..2088210	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MUA67_RS10455 (2088559)	2088559..2088921	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA67_RS10460 (2088918)	2088918..2089088	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA67_RS10465 (2089173)	2089173..2090321	-	1149	WP_001281154.1	alanine racemase	-
MUA67_RS10470 (2090387)	2090387..2090746	-	360	WP_000581202.1	holo-ACP synthase	-
MUA67_RS10475 (2090750)	2090750..2091241	-	492	WP_001205910.1	PH domain-containing protein	-
MUA67_RS10480 (2091228)	2091228..2092811	-	1584	WP_262610202.1	PH domain-containing protein	-
MUA67_RS10485 (2092804)	2092804..2093283	-	480	WP_262610204.1	hypothetical protein	-
MUA67_RS10490 (2093492)	2093492..2094052	-	561	WP_262610206.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240224 WP_000621175.1 NZ_CP094758:c2088921-2088559 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240224 NZ_LS992180:3764884-3765102 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240224 WP_000948331.1 NZ_CP094758:c2089088-2088918 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240224 NZ_LS992180:3764484-3764858 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1