Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2132720..2133249 Replicon chromosome
Accession NZ_CP094755
Organism Staphylococcus aureus strain IVB6242

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MUA32_RS10640 Protein ID WP_000621176.1
Coordinates 2132720..2133082 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MUA32_RS10645 Protein ID WP_000948331.1
Coordinates 2133079..2133249 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA32_RS10620 (2129698) 2129698..2130468 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MUA32_RS10625 (2130443) 2130443..2130922 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MUA32_RS10630 (2130924) 2130924..2131250 - 327 WP_001052491.1 anti-sigma factor antagonist -
MUA32_RS10635 (2131369) 2131369..2132370 - 1002 WP_262515884.1 PP2C family protein-serine/threonine phosphatase -
MUA32_RS10640 (2132720) 2132720..2133082 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUA32_RS10645 (2133079) 2133079..2133249 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MUA32_RS10650 (2133334) 2133334..2134482 - 1149 WP_262515887.1 alanine racemase -
MUA32_RS10655 (2134548) 2134548..2134907 - 360 WP_000581197.1 holo-ACP synthase -
MUA32_RS10660 (2134911) 2134911..2135402 - 492 WP_001286801.1 PH domain-containing protein -
MUA32_RS10665 (2135395) 2135395..2136972 - 1578 WP_262515888.1 PH domain-containing protein -
MUA32_RS10670 (2136965) 2136965..2137444 - 480 WP_262515889.1 hypothetical protein -
MUA32_RS10675 (2137653) 2137653..2138213 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T240201 WP_000621176.1 NZ_CP094755:c2133082-2132720 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T240201 NZ_LS992175:2212228-2212331 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT240201 WP_000948331.1 NZ_CP094755:c2133249-2133079 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT240201 NZ_LS992175:c2212336-2212193 [Citrobacter freundii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References