Detailed information of TA system TA024020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 703496..704139
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P67239
• Locus tag: TBMG_RS03165
• Protein ID: WP_003403187.1
• Coordinates: 703738..704139 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ91
• Locus tag: TBMG_RS03160
• Protein ID: WP_003403184.1
• Coordinates: 703496..703741 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS03125	698776..699306	-	531	WP_003917320.1	two component system sensor kinase HK2	-
TBMG_RS03130	699290..699985	-	696	WP_003918099.1	two-component system response regulator TcrA	-
TBMG_RS03135	700108..700419	+	312	WP_003403164.1	hypothetical protein	-
TBMG_RS03140	700491..701441	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
TBMG_RS03145	701682..702266	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
TBMG_RS03150	702268..702981	+	714	Protein_625	IS607 family element transposase accessory protein TnpB	-
TBMG_RS03155	702981..703451	+	471	WP_003898523.1	hypothetical protein	-
TBMG_RS03160	703496..703741	+	246	WP_003403184.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBMG_RS03165	703738..704139	+	402	WP_003403187.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBMG_RS22020	704527..704694	-	168	WP_077385191.1	DUF3800 domain-containing protein	-
TBMG_RS22025	704727..704924	-	198	WP_003403191.1	hypothetical protein	-
TBMG_RS03180	705004..706161	-	1158	WP_003898524.1	hypothetical protein	-
TBMG_RS03185	706213..706434	-	222	WP_003898525.1	DUF4926 domain-containing protein	-
TBMG_RS03190	706576..707181	+	606	WP_003898526.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14501.21 Da
• Isoelectric Point: 4.4463

>T24020 WP_003403187.1 NC_012943:703738-704139 [Mycobacterium tuberculosis KZN 1435]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Nucleotide

• Length: 402 bp

>T24020 NC_012943:703738-704139 [Mycobacterium tuberculosis KZN 1435]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8813.12 Da
• Isoelectric Point: 10.3486

>AT24020 WP_003403184.1 NC_012943:703496-703741 [Mycobacterium tuberculosis KZN 1435]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Nucleotide

• Length: 246 bp

>AT24020 NC_012943:703496-703741 [Mycobacterium tuberculosis KZN 1435]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSD6

Antitoxin

• AlphaFold DB: G0TQ91