Detailed information of TA system TA240194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 858748..859225
• Replicon: chromosome
• Accession: NZ_CP094755
• Organism: Staphylococcus aureus strain IVB6242

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: MUA32_RS04105
• Protein ID: WP_000052076.1
• Coordinates: 858748..858936 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: MUA32_RS04110
• Protein ID: WP_001059690.1
• Coordinates: 858965..859225 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA32_RS04075 (854701)	854701..855258	+	558	WP_000824491.1	hypothetical protein	-
MUA32_RS04080 (855599)	855599..856285	+	687	WP_001574556.1	thermonuclease family protein	-
MUA32_RS04085 (856642)	856642..856842	+	201	WP_001059082.1	cold-shock protein	-
MUA32_RS04090 (857339)	857339..857557	-	219	WP_000005879.1	hypothetical protein	-
MUA32_RS04095 (857619)	857619..857903	-	285	WP_001004401.1	hypothetical protein	-
MUA32_RS04100 (857991)	857991..858560	-	570	WP_001106663.1	lipoprotein N-acylation protein LnsA	-
MUA32_RS04105 (858748)	858748..858936	-	189	WP_000052076.1	hypothetical protein	Toxin
MUA32_RS04110 (858965)	858965..859225	-	261	WP_001059690.1	hypothetical protein	Antitoxin
MUA32_RS04115 (859566)	859566..859769	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
MUA32_RS04120 (859766)	859766..860002	-	237	WP_000368687.1	hypothetical protein	-
MUA32_RS04125 (860227)	860227..860808	+	582	WP_001140696.1	histidine phosphatase family protein	-
MUA32_RS04130 (860881)	860881..861498	-	618	WP_000255548.1	LysE/ArgO family amino acid transporter	-
MUA32_RS04135 (861654)	861654..862148	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
MUA32_RS04140 (862547)	862547..862969	-	423	WP_000974455.1	organic hydroperoxide resistance protein	-
MUA32_RS04145 (863117)	863117..863833	+	717	WP_000150025.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	853345..885731	32386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T240194 WP_000052076.1 NZ_CP094755:c858936-858748 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T240194 NZ_LS992172:67044-67442 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT240194 WP_001059690.1 NZ_CP094755:c859225-858965 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT240194 NZ_LS992172:66817-67044 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56