Detailed information of TA system TA024019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697406..698052
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: TBMG_RS03110
• Protein ID: WP_003403137.1
• Coordinates: 697406..697819 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: TBMG_RS03115
• Protein ID: WP_003403139.1
• Coordinates: 697816..698052 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS03090	693489..695039	+	1551	WP_003403119.1	MCE family protein	-
TBMG_RS03095	695091..695483	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
TBMG_RS03100	695480..695737	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
TBMG_RS03105	695920..697155	-	1236	WP_003403128.1	ATP-binding protein	-
TBMG_RS03110	697406..697819	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBMG_RS03115	697816..698052	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TBMG_RS03120	698156..698662	-	507	WP_003900973.1	two component system sensor kinase HK1	-
TBMG_RS03125	698776..699306	-	531	WP_003917320.1	two component system sensor kinase HK2	-
TBMG_RS03130	699290..699985	-	696	WP_003918099.1	two-component system response regulator TcrA	-
TBMG_RS03135	700108..700419	+	312	WP_003403164.1	hypothetical protein	-
TBMG_RS03140	700491..701441	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
TBMG_RS03145	701682..702266	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
TBMG_RS03150	702268..702981	+	714	Protein_625	IS607 family element transposase accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T24019 WP_003403137.1 NC_012943:c697819-697406 [Mycobacterium tuberculosis KZN 1435]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T24019 NC_012943:c697819-697406 [Mycobacterium tuberculosis KZN 1435]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT24019 WP_003403139.1 NC_012943:c698052-697816 [Mycobacterium tuberculosis KZN 1435]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT24019 NC_012943:c698052-697816 [Mycobacterium tuberculosis KZN 1435]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89