Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2132717..2133246 Replicon chromosome
Accession NZ_CP094752
Organism Staphylococcus aureus strain IVB6243

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MUA86_RS10640 Protein ID WP_000621176.1
Coordinates 2132717..2133079 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MUA86_RS10645 Protein ID WP_000948331.1
Coordinates 2133076..2133246 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA86_RS10620 (2129695) 2129695..2130465 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MUA86_RS10625 (2130440) 2130440..2130919 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MUA86_RS10630 (2130921) 2130921..2131247 - 327 WP_001052491.1 anti-sigma factor antagonist -
MUA86_RS10635 (2131366) 2131366..2132367 - 1002 WP_262515884.1 PP2C family protein-serine/threonine phosphatase -
MUA86_RS10640 (2132717) 2132717..2133079 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUA86_RS10645 (2133076) 2133076..2133246 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MUA86_RS10650 (2133331) 2133331..2134479 - 1149 WP_262515887.1 alanine racemase -
MUA86_RS10655 (2134545) 2134545..2134904 - 360 WP_000581197.1 holo-ACP synthase -
MUA86_RS10660 (2134908) 2134908..2135399 - 492 WP_001286801.1 PH domain-containing protein -
MUA86_RS10665 (2135392) 2135392..2136969 - 1578 WP_262515888.1 PH domain-containing protein -
MUA86_RS10670 (2136962) 2136962..2137441 - 480 WP_262515889.1 hypothetical protein -
MUA86_RS10675 (2137650) 2137650..2138210 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T240183 WP_000621176.1 NZ_CP094752:c2133079-2132717 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T240183 NZ_LS992171:2648528-2648635 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT240183 WP_000948331.1 NZ_CP094752:c2133246-2133076 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT240183 NZ_LS992171:c2648481-2648415 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References