Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 695091..695737 Replicon chromosome
Accession NC_012943
Organism Mycobacterium tuberculosis KZN 1435

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag TBMG_RS03095 Protein ID WP_003403122.1
Coordinates 695091..695483 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag TBMG_RS03100 Protein ID WP_003403125.1
Coordinates 695480..695737 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBMG_RS03080 690753..692279 + 1527 WP_003403112.1 virulence factor Mce family protein -
TBMG_RS03085 692342..693484 + 1143 WP_003911253.1 virulence factor Mce family protein -
TBMG_RS03090 693489..695039 + 1551 WP_003403119.1 MCE family protein -
TBMG_RS03095 695091..695483 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
TBMG_RS03100 695480..695737 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
TBMG_RS03105 695920..697155 - 1236 WP_003403128.1 ATP-binding protein -
TBMG_RS03110 697406..697819 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
TBMG_RS03115 697816..698052 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
TBMG_RS03120 698156..698662 - 507 WP_003900973.1 two component system sensor kinase HK1 -
TBMG_RS03125 698776..699306 - 531 WP_003917320.1 two component system sensor kinase HK2 -
TBMG_RS03130 699290..699985 - 696 WP_003918099.1 two-component system response regulator TcrA -
TBMG_RS03135 700108..700419 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T24018 WP_003403122.1 NC_012943:c695483-695091 [Mycobacterium tuberculosis KZN 1435]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T24018 NC_012943:c695483-695091 [Mycobacterium tuberculosis KZN 1435]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT24018 WP_003403125.1 NC_012943:c695737-695480 [Mycobacterium tuberculosis KZN 1435]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT24018 NC_012943:c695737-695480 [Mycobacterium tuberculosis KZN 1435]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References