Detailed information of TA system TA240167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2118410..2118939
• Replicon: chromosome
• Accession: NZ_CP094750
• Organism: Staphylococcus aureus strain IVB6248

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA07_RS10400
• Protein ID: WP_000621175.1
• Coordinates: 2118410..2118772 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUA07_RS10405
• Protein ID: WP_000948331.1
• Coordinates: 2118769..2118939 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA07_RS10380 (MUA07_10380)	2115388..2116158	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
MUA07_RS10385 (MUA07_10385)	2116133..2116612	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
MUA07_RS10390 (MUA07_10390)	2116614..2116940	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MUA07_RS10395 (MUA07_10395)	2117059..2118060	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MUA07_RS10400 (MUA07_10400)	2118410..2118772	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA07_RS10405 (MUA07_10405)	2118769..2118939	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA07_RS10410 (MUA07_10410)	2119024..2120172	-	1149	WP_001281160.1	alanine racemase	-
MUA07_RS10415 (MUA07_10415)	2120238..2120597	-	360	WP_000581198.1	holo-ACP synthase	-
MUA07_RS10420 (MUA07_10420)	2120601..2121092	-	492	WP_001205919.1	PH domain-containing protein	-
MUA07_RS10425 (MUA07_10425)	2121079..2122662	-	1584	WP_252550166.1	PH domain-containing protein	-
MUA07_RS10430 (MUA07_10430)	2122655..2123134	-	480	WP_001287083.1	hypothetical protein	-
MUA07_RS10435 (MUA07_10435)	2123343..2123903	-	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240167 WP_000621175.1 NZ_CP094750:c2118772-2118410 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240167 NZ_LS992169:c109783-109478 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240167 WP_000948331.1 NZ_CP094750:c2118939-2118769 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240167 NZ_LS992169:c110003-109785 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1