Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2167886..2168415 Replicon chromosome
Accession NZ_CP094747
Organism Staphylococcus aureus strain IVB6249

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MUA08_RS10855 Protein ID WP_000621176.1
Coordinates 2167886..2168248 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MUA08_RS10860 Protein ID WP_000948331.1
Coordinates 2168245..2168415 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA08_RS10835 (MUA08_10835) 2164864..2165634 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MUA08_RS10840 (MUA08_10840) 2165609..2166088 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MUA08_RS10845 (MUA08_10845) 2166090..2166416 - 327 WP_001052491.1 anti-sigma factor antagonist -
MUA08_RS10850 (MUA08_10850) 2166535..2167536 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
MUA08_RS10855 (MUA08_10855) 2167886..2168248 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUA08_RS10860 (MUA08_10860) 2168245..2168415 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MUA08_RS10865 (MUA08_10865) 2168500..2169648 - 1149 WP_001281140.1 alanine racemase -
MUA08_RS10870 (MUA08_10870) 2169714..2170073 - 360 WP_000581197.1 holo-ACP synthase -
MUA08_RS10875 (MUA08_10875) 2170077..2170568 - 492 WP_001286801.1 PH domain-containing protein -
MUA08_RS10880 (MUA08_10880) 2170561..2172138 - 1578 WP_001294646.1 PH domain-containing protein -
MUA08_RS10885 (MUA08_10885) 2172131..2172610 - 480 WP_001287082.1 hypothetical protein -
MUA08_RS10890 (MUA08_10890) 2172819..2173379 - 561 WP_262518315.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T240155 WP_000621176.1 NZ_CP094747:c2168248-2167886 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T240155 NZ_LS992168:c3631001-3630699 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT240155 WP_000948331.1 NZ_CP094747:c2168415-2168245 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT240155 NZ_LS992168:c3630663-3630322 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References