Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2154327..2154856 Replicon chromosome
Accession NZ_CP094745
Organism Staphylococcus aureus strain IVB6250

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MUA64_RS10755 Protein ID WP_000621176.1
Coordinates 2154327..2154689 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MUA64_RS10760 Protein ID WP_000948331.1
Coordinates 2154686..2154856 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA64_RS10735 (MUA64_10735) 2151305..2152075 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MUA64_RS10740 (MUA64_10740) 2152050..2152529 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MUA64_RS10745 (MUA64_10745) 2152531..2152857 - 327 WP_001052491.1 anti-sigma factor antagonist -
MUA64_RS10750 (MUA64_10750) 2152976..2153977 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
MUA64_RS10755 (MUA64_10755) 2154327..2154689 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MUA64_RS10760 (MUA64_10760) 2154686..2154856 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MUA64_RS10765 (MUA64_10765) 2154941..2156089 - 1149 WP_001281140.1 alanine racemase -
MUA64_RS10770 (MUA64_10770) 2156155..2156514 - 360 WP_000581197.1 holo-ACP synthase -
MUA64_RS10775 (MUA64_10775) 2156518..2157009 - 492 WP_001286801.1 PH domain-containing protein -
MUA64_RS10780 (MUA64_10780) 2157002..2158579 - 1578 WP_001294646.1 PH domain-containing protein -
MUA64_RS10785 (MUA64_10785) 2158572..2159051 - 480 WP_001287082.1 hypothetical protein -
MUA64_RS10790 (MUA64_10790) 2159260..2159820 - 561 WP_262518315.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T240143 WP_000621176.1 NZ_CP094745:c2154689-2154327 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T240143 NZ_LS992168:186023-186130 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT240143 WP_000948331.1 NZ_CP094745:c2154856-2154686 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT240143 NZ_LS992168:c185966-185909 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References