Detailed information of TA system TA240130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2118275..2118804
• Replicon: chromosome
• Accession: NZ_CP094743
• Organism: Staphylococcus aureus strain IVB6252

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA62_RS10400
• Protein ID: WP_000621175.1
• Coordinates: 2118275..2118637 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUA62_RS10405
• Protein ID: WP_000948331.1
• Coordinates: 2118634..2118804 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA62_RS10380 (MUA62_10380)	2115253..2116023	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
MUA62_RS10385 (MUA62_10385)	2115998..2116477	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
MUA62_RS10390 (MUA62_10390)	2116479..2116805	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MUA62_RS10395 (MUA62_10395)	2116924..2117925	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MUA62_RS10400 (MUA62_10400)	2118275..2118637	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA62_RS10405 (MUA62_10405)	2118634..2118804	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA62_RS10410 (MUA62_10410)	2118889..2120037	-	1149	WP_001281160.1	alanine racemase	-
MUA62_RS10415 (MUA62_10415)	2120103..2120462	-	360	WP_000581198.1	holo-ACP synthase	-
MUA62_RS10420 (MUA62_10420)	2120466..2120957	-	492	WP_001205919.1	PH domain-containing protein	-
MUA62_RS10425 (MUA62_10425)	2120944..2122527	-	1584	WP_252550166.1	PH domain-containing protein	-
MUA62_RS10430 (MUA62_10430)	2122520..2122999	-	480	WP_001287083.1	hypothetical protein	-
MUA62_RS10435 (MUA62_10435)	2123208..2123768	-	561	WP_001092403.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T240130 WP_000621175.1 NZ_CP094743:c2118637-2118275 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T240130 NZ_LS992166:c2547439-2547263 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT240130 WP_000948331.1 NZ_CP094743:c2118804-2118634 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT240130 NZ_LS992166:c2547217-2546801 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1