Detailed information of TA system TA240121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 718075..718599
• Replicon: chromosome
• Accession: NZ_CP094742
• Organism: Staphylococcus chromogenes strain IVB6199

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA20_RS03500
• Protein ID: WP_103159488.1
• Coordinates: 718246..718599 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A3A0K2V6
• Locus tag: MUA20_RS03495
• Protein ID: WP_037577177.1
• Coordinates: 718075..718245 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA20_RS03470 (MUA20_03450)	713923..714396	+	474	WP_262621704.1	PH domain-containing protein	-
MUA20_RS03475 (MUA20_03455)	714389..715867	+	1479	WP_262621705.1	PH domain-containing protein	-
MUA20_RS03480 (MUA20_03460)	715864..716379	+	516	WP_051605021.1	PH domain-containing protein	-
MUA20_RS03485 (MUA20_03465)	716376..716726	+	351	WP_262621706.1	holo-ACP synthase	-
MUA20_RS03490 (MUA20_03470)	716842..717990	+	1149	WP_107372183.1	alanine racemase	-
MUA20_RS03495 (MUA20_03475)	718075..718245	+	171	WP_037577177.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA20_RS03500 (MUA20_03480)	718246..718599	+	354	WP_103159488.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA20_RS03505 (MUA20_03485)	718663..719667	+	1005	WP_262621707.1	PP2C family protein-serine/threonine phosphatase	-
MUA20_RS03510 (MUA20_03490)	719748..720074	+	327	WP_037577172.1	anti-sigma factor antagonist	-
MUA20_RS03515 (MUA20_03495)	720077..720556	+	480	WP_037577169.1	anti-sigma B factor RsbW	-
MUA20_RS03520 (MUA20_03500)	720531..721301	+	771	WP_037577167.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13064.34 Da
• Isoelectric Point: 10.5127

>T240121 WP_103159488.1 NZ_CP094742:718246-718599 [Staphylococcus chromogenes]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIDKKKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSDKKMKEVNAALGISLGLHMNQHK

Nucleotide

• Length: 354 bp

>T240121 NZ_LS992166:734920-735216 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6328.01 Da
• Isoelectric Point: 4.2313

>AT240121 WP_037577177.1 NZ_CP094742:718075-718245 [Staphylococcus chromogenes]
MSAFNQTRVKSLEQSLKEGYVQMADLNLSLATEAYFVECEACDCNESHLLSNHKDD

Nucleotide

• Length: 171 bp

>AT240121 NZ_LS992166:735218-735613 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3A0K2V6