Detailed information of TA system TA240035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 846568..847092
• Replicon: chromosome
• Accession: NZ_CP094712
• Organism: Staphylococcus schleiferi strain IVB6183

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S2X8B9
• Locus tag: MUA40_RS03810
• Protein ID: WP_016426488.1
• Coordinates: 846739..847092 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S2XCM0
• Locus tag: MUA40_RS03805
• Protein ID: WP_016426487.1
• Coordinates: 846568..846738 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA40_RS03780 (MUA40_03770)	842496..842924	+	429	WP_167512901.1	PH domain-containing protein	-
MUA40_RS03785 (MUA40_03775)	842917..844431	+	1515	WP_155976489.1	PH domain-containing protein	-
MUA40_RS03790 (MUA40_03780)	844415..844936	+	522	WP_126496614.1	PH domain-containing protein	-
MUA40_RS03795 (MUA40_03785)	844933..845289	+	357	WP_016426485.1	holo-ACP synthase	-
MUA40_RS03800 (MUA40_03790)	845334..846482	+	1149	WP_262569915.1	alanine racemase	-
MUA40_RS03805 (MUA40_03795)	846568..846738	+	171	WP_016426487.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA40_RS03810 (MUA40_03800)	846739..847092	+	354	WP_016426488.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA40_RS03815 (MUA40_03805)	847151..848155	+	1005	WP_262569916.1	PP2C family protein-serine/threonine phosphatase	-
MUA40_RS03820 (MUA40_03810)	848234..848560	+	327	WP_016426490.1	anti-sigma factor antagonist	-
MUA40_RS03825 (MUA40_03815)	848562..849041	+	480	WP_167732819.1	anti-sigma B factor RsbW	-
MUA40_RS03830 (MUA40_03820)	849016..849786	+	771	WP_016426492.1	RNA polymerase sigma factor SigB	-
MUA40_RS03835 (MUA40_03825)	850373..851578	-	1206	WP_081635139.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13157.33 Da
• Isoelectric Point: 10.1660

>T240035 WP_016426488.1 NZ_CP094712:846739-847092 [Staphylococcus schleiferi]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEEKMKEVNYALGISLGLNMNHHK

Nucleotide

• Length: 354 bp

>T240035 NZ_LS483472:455826-456215 [Acinetobacter baumannii]
TTGCTCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATAAGCTTTATTTTCTTTT
TAAAGCGTCCGCAACTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6370.00 Da
• Isoelectric Point: 3.9760

>AT240035 WP_016426487.1 NZ_CP094712:846568-846738 [Staphylococcus schleiferi]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYSVECEACDCNESHLLSDYKDD

Nucleotide

• Length: 171 bp

>AT240035 NZ_LS483472:455562-455819 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: S2X8B9

Antitoxin

• AlphaFold DB: S2XCM0