Detailed information of TA system TA240032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 848109..848633
• Replicon: chromosome
• Accession: NZ_CP094711
• Organism: Staphylococcus schleiferi strain IVB6184

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S2X8B9
• Locus tag: MUA46_RS03805
• Protein ID: WP_016426488.1
• Coordinates: 848280..848633 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S2XCM0
• Locus tag: MUA46_RS03800
• Protein ID: WP_016426487.1
• Coordinates: 848109..848279 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA46_RS03775 (MUA46_03765)	844037..844465	+	429	WP_167512901.1	PH domain-containing protein	-
MUA46_RS03780 (MUA46_03770)	844458..845972	+	1515	WP_155976489.1	PH domain-containing protein	-
MUA46_RS03785 (MUA46_03775)	845956..846477	+	522	WP_126496614.1	PH domain-containing protein	-
MUA46_RS03790 (MUA46_03780)	846474..846830	+	357	WP_016426485.1	holo-ACP synthase	-
MUA46_RS03795 (MUA46_03785)	846875..848023	+	1149	WP_262576521.1	alanine racemase	-
MUA46_RS03800 (MUA46_03790)	848109..848279	+	171	WP_016426487.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA46_RS03805 (MUA46_03795)	848280..848633	+	354	WP_016426488.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA46_RS03810 (MUA46_03800)	848692..849696	+	1005	WP_016426489.1	PP2C family protein-serine/threonine phosphatase	-
MUA46_RS03815 (MUA46_03805)	849775..850101	+	327	WP_016426490.1	anti-sigma factor antagonist	-
MUA46_RS03820 (MUA46_03810)	850103..850582	+	480	WP_167732819.1	anti-sigma B factor RsbW	-
MUA46_RS03825 (MUA46_03815)	850557..851327	+	771	WP_016426492.1	RNA polymerase sigma factor SigB	-
MUA46_RS03830 (MUA46_03820)	851914..853119	-	1206	WP_262576556.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13157.33 Da
• Isoelectric Point: 10.1660

>T240032 WP_016426488.1 NZ_CP094711:848280-848633 [Staphylococcus schleiferi]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEEKMKEVNYALGISLGLNMNHHK

Nucleotide

• Length: 354 bp

>T240032 NZ_LS483470:3382537-3383469 [Leminorella richardii]
ATGGATCTGCGTGATAGTTATGCCCGGCAGGTCAGGCTATTAATGGCTGCCCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCATTGAAAGGCGGCACAGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATCGATCTGGCGTATA
AGACCTTTGCGGATCGCGATACCGATTTGGCAGCTATCAACGATGCTTTGAAGAGAATTACTGCATCGCTGAACAGTAAA
CCGGGGATCACCGCGATTCGACAGGAAAATAAAGCGGATGAGAAGCGCATCATTGTGAATACCACTGATGCACAGATAAA
GATCGAGGTTAGTCCAGTCTGGCGCGGGCTGCTGCTGCCACCAACAGAAATGCCAGTATGTGAACAGGTGGAAATGGAAT
ATGGATTCACCACCATGAATGTCGTTTCGCTGGCAGATCTTTATGGCGGTAAAATCTGCGCCGCGCTCGATCGCCAGCAT
CCGCGCGATCTGTTTGACGTGCTAAATATGCTGAAAAAACCGGGCCTGACGCGCGAGATCTTTGACGGTTTTCTTTGCTA
TCTGGCAGGCCATCCTCGCCCCATTGCTGAGTTACTGACTCCCAACTGGGATACAGCGCGGATCGCCACATTGTACGCAC
AGGAATTTTCAGGAATGACAACACAGCGGGAAACATCGCTGGAGTCGCTTTTATCAGTAACGACGTTGTTGCCACAAGCA
CTGAAATCGCATCTTACGGACAGGGATCGCCAGTTCTTACTCAGTTATAAGCAAAATACCCCTGACTGGTCGCTGTATCG
CTATCCTGAAATTCAACACCTACCGGCTATTCGCTGGAAGCTGCGTAATCTGGCCGTACTGAAGGAAAAGAACCCGGCCA
AGAATACGGCGGCGGTGAGCAAGTTAGAAGGAGTTCTGGAGCAGTACTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6370.00 Da
• Isoelectric Point: 3.9760

>AT240032 WP_016426487.1 NZ_CP094711:848109-848279 [Staphylococcus schleiferi]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYSVECEACDCNESHLLSDYKDD

Nucleotide

• Length: 171 bp

>AT240032 NZ_LS483470:3381768-3382550 [Leminorella richardii]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACCCTCCCTGGTCAGGTGCTGCTCCAATCATGGCTTACCCGTAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTCCAGAATGGCTGGCTCAGACGAATCGCTCACGGCGTTTACTGCCGGACGG
GTCGCGATCCAGACTGGGTGGATGCGGTTTACTGCCTGCAAACGCAGTGGGAAAAACCGGTTCGTGTAGCGGGGCTGACC
AGCCTTGCACTACAGGGCCACGCTCACTATACCGAGCCGGGCAGACAACAAATCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTCGCCACCTTTGAACAATCCGCGACGTTTGTCACGCTGTATACGTCAGGCCTTACCGTTGATATCA
CGTTATTGACCACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGTCCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGTGTTCCTTCGTTGATCTCGTTCGAGCATGCCGATGCTCTGTTTCAGGGAATGAATCTTCTCAGTCCGCGTAAATT
GCAAACACTGCTCTGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTCTATCTTGCCCGGCGCAATGAGCATCCTT
ATTTTCGGTATCTCGATCAAAGCGGTATAGAAACAGGTACGGGAAAACGGCAAATCGTTCCCGGCGGCTGGCTGGAACCG
GATTATCAAATCACCGTTCCCCGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: S2X8B9

Antitoxin

• AlphaFold DB: S2XCM0