Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 963573..964087 Replicon chromosome
Accession NZ_CP094709
Organism Staphylococcus simulans strain IVB6174

Toxin (Protein)


Gene name TsdT Uniprot ID A0A133QQ97
Locus tag MUA82_RS04335 Protein ID WP_002481764.1
Coordinates 963881..964087 (+) Length 69 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A133QQA1
Locus tag MUA82_RS04330 Protein ID WP_002481765.1
Coordinates 963573..963866 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA82_RS04305 (MUA82_04305) 958967..959605 + 639 WP_262583271.1 thiamine phosphate synthase -
MUA82_RS04310 (MUA82_04310) 959694..960563 + 870 WP_002481769.1 membrane protein insertase YidC -
MUA82_RS04315 (MUA82_04315) 960689..961201 + 513 WP_002481768.1 GNAT family N-acetyltransferase -
MUA82_RS04320 (MUA82_04320) 961259..961909 - 651 WP_023016109.1 HD domain-containing protein -
MUA82_RS04325 (MUA82_04325) 961933..963414 - 1482 WP_262583272.1 cardiolipin synthase -
MUA82_RS04330 (MUA82_04330) 963573..963866 + 294 WP_002481765.1 copper-sensing transcriptional repressor CsoR Antitoxin
MUA82_RS04335 (MUA82_04335) 963881..964087 + 207 WP_002481764.1 heavy metal-associated domain-containing protein Toxin
MUA82_RS04340 (MUA82_04340) 964106..964312 + 207 WP_002481763.1 copper ion binding protein -
MUA82_RS04345 (MUA82_04345) 964405..966597 + 2193 WP_262583273.1 heavy metal translocating P-type ATPase -
MUA82_RS04350 (MUA82_04350) 966679..966819 + 141 WP_023016106.1 Lmo0850 family protein -
MUA82_RS04355 (MUA82_04355) 966879..968087 - 1209 WP_002481760.1 FtsW/RodA/SpoVE family cell cycle protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(24-65)

Antitoxin

Metal-sensitive transcriptional repressor

(3-95)


Sequences

Toxin        


Download         Length: 69 a.a.        Molecular weight: 7861.82 Da        Isoelectric Point: 4.2670

>T240030 WP_002481764.1 NZ_CP094709:963881-964087 [Staphylococcus simulans]
MHSSVIQISGMETEEQQHILQEHLQQMIGMSEVQVDLKSQEVKASYQTPISLNNLEKEIYDQGYQILN

Download         Length: 207 bp

>T240030 NZ_LS483470:1628137-1628412 [Leminorella richardii]
ATGCAAGAAAAGGTTCTATTGCTTAGGAAAAAGCAGTATTCCACCCTGTCGCAACTTTTTAAGATCCCTGCTTTGGCAGG
GATAAAATGGAAAGATATTGAGTCGTTGGTGATTGCTCTCGGCGGTGAAGTTCAGGAGGGGAATGGCTCAAGGGTAAAGT
TCTTTCTGGCTGGCAGCATTGCGCATTTTCACCGTCCGCATCCGTCACCCGATACGGATAAAGGCGCTGTTGTCAGCGTT
GGTGATTGGCTTATGAGTTTGGGGGTAACGCCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11033.69 Da        Isoelectric Point: 6.9688

>AT240030 WP_002481765.1 NZ_CP094709:963573-963866 [Staphylococcus simulans]
MTQEERAHHSEKIKSNLKARLNRIEGQVRAINRMIDEDVYCDDVLTQIRATRSALNSVAVKLLDHHMKGCILHKAEDGQA
EAAMEELLVTVQKLIKD

Download         Length: 294 bp

>AT240030 NZ_LS483470:1628415-1628783 [Leminorella richardii]
ATGAAGAAACAGCCTGATATTAATACACTTAATACCATGGTTGTGGCCGGTCAGCCGGCTATTATTAGTTTTGTTCCGGA
GCTGGGAGCTTTTCGCGGTCGCTTTCTTGGCCTGTCAGGCTACTGTGACTTTGTCGCTGACAGCATTAGCGCATTAAAGC
ATGAAGGTGAAATCTCTTTACGTGAATATCTGGAAGACTGCAAAGAGCATGGGATAGAGCCGTATGCCCGAAAGGAAAAG
CAGAGGACATTTACGCTACGCTATCCAGAATCGTTTGGCGAACAGTTAGAGCACGCCGCTGCCGCGTCTGAAGTTTCGCT
GAACTCGTTCATTGTTGACGTTTTGAAAGAGCGGCTGTCGCGGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133QQ97

Antitoxin

Source ID Structure
AlphaFold DB A0A133QQA1

References