Detailed information of TA system TA240022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1035491..1036005
• Replicon: chromosome
• Accession: NZ_CP094704
• Organism: Staphylococcus simulans strain IVB6207

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA54_RS04595
• Protein ID: WP_031262808.1
• Coordinates: 1035655..1036005 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2T4QNU3
• Locus tag: MUA54_RS04590
• Protein ID: WP_023016097.1
• Coordinates: 1035491..1035658 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA54_RS04565 (MUA54_04555)	1031381..1031845	+	465	WP_105967036.1	PH domain-containing protein	-
MUA54_RS04570 (MUA54_04560)	1031838..1033331	+	1494	WP_023016101.1	PH domain-containing protein	-
MUA54_RS04575 (MUA54_04565)	1033324..1033821	+	498	WP_262554957.1	PH domain-containing protein	-
MUA54_RS04580 (MUA54_04570)	1033823..1034176	+	354	WP_031262810.1	holo-ACP synthase	-
MUA54_RS04585 (MUA54_04575)	1034256..1035410	+	1155	WP_023016098.1	alanine racemase	-
MUA54_RS04590 (MUA54_04580)	1035491..1035658	+	168	WP_023016097.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA54_RS04595 (MUA54_04585)	1035655..1036005	+	351	WP_031262808.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA54_RS04600 (MUA54_04590)	1036297..1037298	+	1002	WP_060804063.1	SpoIIE family protein phosphatase	-
MUA54_RS04605 (MUA54_04595)	1037374..1037700	+	327	WP_002481749.1	anti-sigma factor antagonist	-
MUA54_RS04610 (MUA54_04600)	1037702..1038181	+	480	WP_023016096.1	anti-sigma B factor RsbW	-
MUA54_RS04615 (MUA54_04605)	1038156..1038926	+	771	WP_002481747.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.09 Da
• Isoelectric Point: 10.0474

>T240022 WP_031262808.1 NZ_CP094704:1035655-1036005 [Staphylococcus simulans]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIGISLGLTHKN

Nucleotide

• Length: 351 bp

>T240022 NZ_LS483458:1597372-1598652 [Haemophilus haemolyticus]
ATGAGTAACTCGATTCTAAACGTTGCCATGAATGGCTTGTTAGTCGGTGAATGGCGAAAACTCACCAGTGGCGCAACCGA
ATTTCAGTATGCGGAAAAATGGCTTGAATCTGTTCGCTCGCGCCCTATTTCATTGTCACTGCCCTTGTCGCACAAAATTT
ATCGAGGAGATTCCGTTTACAATTTCTTCGATAATCTCCTCCCCGACAATGAACAGATCCGTTCACGCATTCAACAACGT
TTCCAAACATCAACGAAATCCCCATTTGATTTGCTTTCCGCGATTGGACAGGATTGTATTGGCGCAATTCAGCTTTATCA
GGGGCAGAGCCGCTCGGTACGTCAAATTTATGCAGAACCACTAAATGACAAAGAGGTGGAAAACGTGCTGAAAAACTACC
GCACTTTTCCTCTAGGCATGGAAGAAAGCGAGGATTTCCGAATTTCTTTGGCCGGTGCTCAGGAAAAAACAGCGCTACTT
TATTATCAGAATCAATGGCAACGCCCTTTGCATTCCACCGCGACCAGTCATATTTTTAAACTCCCGATTGGTATGGTGGC
ACAAGGGCAGCTTGATCTTTCCGGCAGCTGTGAAAATGAATGGCTTTGCTTGAATATTATGAAAGCCTTCGGTCTGCTGG
TTCCTCGAAGTCAAATTCAGTATTTCGGTGAAATGAAAGTGCTGATTGTGGAGCGCTTTGATCGCCGTTGGTCTGAAAAT
AGGAGTTGGATAATTCGTTTACCACAAGAAGATATGTGCCAATCACTCAACATCGCCCCTGCGCGAAAATATGAAAATGA
CGGCGGGCCGAGCATTCTTCAAATTATGTCGCTTCTCAACGGTTCATCCAACGCCCAAGCAGATCGCAAGCAGTTTTTTA
AAGCGCAAATTGTTTTCTGGCTACTTTGTGCCATTGACGGCCATGGCAAAAATTTCAGTTTATTTTTGGAACAAGAGAAT
CGCTATCGTCTAACACCTTTTTATGACGTGATGTCCGCCTATCCACTCATCAAACACAACGGACTACAAAAGCAAAAAGT
CAAAATGGCAATGGCGTGGCACGGCGAAAATAAACATTATTTATGGGATAAAATACAGCTCCGCCACATTTTCAACACCG
CCAAACTCACGGGCTTGGCAACGGAAACTGTAGAAAATATTTTGCATGAGATCAGTGCTCTATATCCAAGAGTCAGTGAA
ATAAATACCCATGGATTGCCTGATGAAATTGTGGAGCCAATTTTAATGGGATTGGAAATGCAGATGAAAAAGATAAGTTA
A

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6257.78 Da
• Isoelectric Point: 3.6836

>AT240022 WP_023016097.1 NZ_CP094704:1035491-1035658 [Staphylococcus simulans]
MSTFNQNRSYSLEQSLKEGYSQMADLNLSLATEAFSVECEACDCNESYLTFDKDE

Nucleotide

• Length: 168 bp

>AT240022 NZ_LS483458:1597094-1597372 [Haemophilus haemolyticus]
ATGGTTATCACCACCGCAAAAATGCTTTCTCATGTAATTCGTGAATTTCGCTATCAAGGCGATCTCTCACAAACTGATGT
TGCTAAACTCTCCGGAACGACCCAAGCCAGAATTTCAGCGTTTGAGAATGAGCCGGAGCGTACTAAATTAGAAACTCTAT
TTAAAATTCTGGCAGGATTAGAACTAGAGCTCATTGTGCAACCACGCAAAAAGTCATCTGATACTGCCCGTTCAATCAAG
GAGCCTACAGCAACATACGATGACGACGAGGCATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2T4QNU3