Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 1022194..1022708 Replicon chromosome
Accession NZ_CP094704
Organism Staphylococcus simulans strain IVB6207

Toxin (Protein)


Gene name TsdT Uniprot ID A0A133QQ97
Locus tag MUA54_RS04525 Protein ID WP_002481764.1
Coordinates 1022502..1022708 (+) Length 69 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A133QQA1
Locus tag MUA54_RS04520 Protein ID WP_002481765.1
Coordinates 1022194..1022487 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUA54_RS04495 (MUA54_04485) 1017589..1018227 + 639 WP_002481770.1 thiamine phosphate synthase -
MUA54_RS04500 (MUA54_04490) 1018316..1019185 + 870 WP_002481769.1 membrane protein insertase YidC -
MUA54_RS04505 (MUA54_04495) 1019311..1019823 + 513 WP_002481768.1 GNAT family N-acetyltransferase -
MUA54_RS04510 (MUA54_04500) 1019880..1020530 - 651 WP_023016109.1 HD domain-containing protein -
MUA54_RS04515 (MUA54_04505) 1020554..1022035 - 1482 WP_002481766.1 cardiolipin synthase -
MUA54_RS04520 (MUA54_04510) 1022194..1022487 + 294 WP_002481765.1 copper-sensing transcriptional repressor CsoR Antitoxin
MUA54_RS04525 (MUA54_04515) 1022502..1022708 + 207 WP_002481764.1 heavy metal-associated domain-containing protein Toxin
MUA54_RS04530 (MUA54_04520) 1022727..1022933 + 207 WP_002481763.1 copper ion binding protein -
MUA54_RS04535 (MUA54_04525) 1023026..1025218 + 2193 WP_262554953.1 heavy metal translocating P-type ATPase -
MUA54_RS04540 (MUA54_04530) 1025300..1025440 + 141 WP_023016106.1 Lmo0850 family protein -
MUA54_RS04545 (MUA54_04535) 1025500..1026708 - 1209 WP_002481760.1 FtsW/RodA/SpoVE family cell cycle protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(24-65)

Antitoxin

Metal-sensitive transcriptional repressor

(3-95)


Sequences

Toxin        


Download         Length: 69 a.a.        Molecular weight: 7861.82 Da        Isoelectric Point: 4.2670

>T240021 WP_002481764.1 NZ_CP094704:1022502-1022708 [Staphylococcus simulans]
MHSSVIQISGMETEEQQHILQEHLQQMIGMSEVQVDLKSQEVKASYQTPISLNNLEKEIYDQGYQILN

Download         Length: 207 bp

>T240021 NZ_LS483452:c4152280-4152029 [Shewanella benthica]
ATGGATGCCATTAGCTATACATCTGCTCGAGCCAATTTAGCCTCTACTATGGCGCAAGTTTGTAATGATCACGCACCAAT
CATTATTACGCGAAAAAGTGAGGCTCCCGTTGTAATGATGTCTCTTGAAGATTATCAAGCAATGGAAGAAACAACATACT
TACTCCGTTCGCCTGCCAATGCAAGATATTTGCTTGAGTCAATTGCGGAACTTGAAGCAGGCAAAGGTACCGAACGAGAG
CTGCTTGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11033.69 Da        Isoelectric Point: 6.9688

>AT240021 WP_002481765.1 NZ_CP094704:1022194-1022487 [Staphylococcus simulans]
MTQEERAHHSEKIKSNLKARLNRIEGQVRAINRMIDEDVYCDDVLTQIRATRSALNSVAVKLLDHHMKGCILHKAEDGQA
EAAMEELLVTVQKLIKD

Download         Length: 294 bp

>AT240021 NZ_LS483452:c4152032-4151772 [Shewanella benthica]
ATGAAATTAACGTTTGCAACAAAAGCATGGGAAAATTATCTGTACTGGCAAACTACCGATAAAAAAATGTTAAAGAGAAT
TAATACGTTAATCAAAGAAATTCAACGATCGCCTTATAGCGGTATTGGTAAACCCGAACCACTTAAACACGGGTTAGCTG
GTTACTGGTCTCGACGCATCAATGATGAGCACCGTATAGTGTATAAATACCAAGATGATACGATTCTCATTGCTCAACTG
CGATATCACTACAGCACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133QQ97

Antitoxin

Source ID Structure
AlphaFold DB A0A133QQA1

References