Detailed information of TA system TA239972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/DinJ(antitoxin)
• Location: 1580777..1581416
• Replicon: chromosome
• Accession: NZ_CP094658
• Organism: Bifidobacterium longum subsp. infantis strain JRPT

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W6EZW1
• Locus tag: MT990_RS07310
• Protein ID: WP_014484977.1
• Coordinates: 1581060..1581416 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MT990_RS07305
• Protein ID: WP_243821389.1
• Coordinates: 1580777..1581073 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MT990_RS07280 (MT990_07280)	1575851..1576306	-	456	WP_174773041.1	Holliday junction resolvase RuvX	-
MT990_RS07285 (MT990_07285)	1576315..1578993	-	2679	WP_174773040.1	alanine--tRNA ligase	-
MT990_RS07290 (MT990_07290)	1579122..1579358	-	237	WP_007053386.1	hypothetical protein	-
MT990_RS07295 (MT990_07295)	1579358..1579756	-	399	WP_029679571.1	DUF948 domain-containing protein	-
MT990_RS07300 (MT990_07300)	1579839..1580513	-	675	WP_015438748.1	histidine phosphatase family protein	-
MT990_RS07305 (MT990_07305)	1580777..1581073	+	297	WP_243821389.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MT990_RS07310 (MT990_07310)	1581060..1581416	+	357	WP_014484977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MT990_RS07315 (MT990_07315)	1581593..1582195	-	603	WP_174773039.1	transglutaminase family protein	-
MT990_RS07320 (MT990_07320)	1582300..1582656	-	357	WP_003833231.1	SdpI family protein	-
MT990_RS07325 (MT990_07325)	1582747..1583232	+	486	WP_014483808.1	cytidine deaminase	-
MT990_RS07330 (MT990_07330)	1583370..1584152	+	783	WP_174773038.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
MT990_RS07335 (MT990_07335)	1584331..1584957	-	627	WP_007052130.1	30S ribosomal protein S4	-
MT990_RS07340 (MT990_07340)	1585156..1586148	-	993	WP_174773643.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (12-54)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13302.25 Da
• Isoelectric Point: 8.9603

>T239972 WP_014484977.1 NZ_CP094658:1581060-1581416 [Bifidobacterium longum subsp. infantis]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T239972 NZ_LS483422:c4280820-4279501 [Providencia heimbachae]
ATGGCGACTTTGCATGTGTATATGAATGGTTATCTTGTGGGTGAATTTATTCGCTTAAGTACTGGAGCGCATCAATTTAA
ATATGATATTCACTGGTTGGAAACGCCGGGTGCTCGTCCTATCTCTTTATCAATGCCACTTCAGCATCAAGAATACAAAG
GGGACGAAGTTTATAATTTTTTTGATAACTTATTGCCTGATAATTCAGAGATCCGAAAGCGGGTTGTTAGGCGATATAGA
GCGGAATCGACACAGCCTTTTGATTTACTATCCAAGATAGGACAAGACAGTGTTGGTGCATTACAACTTGTTCCTAACGA
ACTTAATGTTAGCGATATTAAAAAAATAGAATATCGACCATTATCCGATGAAGCGTTGGAAAAAGTATTAAACGGATATA
AATCAGATAGTCCACTCGGAATGATTGAAGAGGTTGATGATTTTAGGATTTCTATTGCAGGGGCTCAAGAAAAAACTGCC
TTACTTTATTTCAATGACCAGTGGTACTTGCCACTTAGAACTACGCCTACAACCCACATTATCAAATTGCCTATAGGTGA
AATACAAAGTCATTCTCATACTCTTGATATGTCAGACAGTGTAGAAAATGAATTTCTATGTTTATTGATAGCCGCAGAGT
TTGGTTTTGATGTGCCTAAATGCACTATTATCAATGTGGGAGAAACGAAGGCACTTGCGGTTGAACGGTTTGACCGTAAA
TTTTCGGCCGATAACGCATGGATTATGCGTTTACCCCAAGAAGACTTCTGCCAAACGTTAAATGTGCTCTCTGAAAATAA
ATATCAAAAGGATGGCGGTCCAGGGATTTACGACATAATGACTTATCTTTTAGGGTCAATAAATACAGAGAAAGATAGAT
ATCAATTTATGAAGAGCCAAGTTCTATTTTGGTTACTTGCTGCCACTGATGGACATGCTAAAAACTTCTCATTATTTATT
GAACCTGAAGGGCGTTATAAATTAACACCTTTTTACGATATTTTGTCAGTATATCCCGTATTTGGCGGTAAAGGACTTAA
TCCAAGAGAGGCAAAATTAGCCATGAGCTTAAAAGGCACGAAAGGCAATAAGGATAAAATAGAACAGATTTTTCCAAGAC
ATTTTTTCGCGACAGCTAAAGCGGTTGGATTTGACCGTACTGAAATGGAAAGAATATTCATTGAGTTTGATGAGCAAATG
GAAAGTGTCATTACGAAAGTCAGGGATCAACTTCCTGCTGATTTTCCTGAACATATTGCAAACTCAATTTTGTCGGGATT
ACAGAGCAAAGCAATACGATTAAAGAAAGGGTGGGATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10996.56 Da
• Isoelectric Point: 4.4919

>AT239972 WP_243821389.1 NZ_CP094658:1580777-1581073 [Bifidobacterium longum subsp. infantis]
MMAIKKKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Nucleotide

• Length: 297 bp

>AT239972 NZ_LS483422:c4281077-4280820 [Providencia heimbachae]
ATGAAAGTCACCAATACTCGGCAACTTAGTGCTTACATGAAAGATGTCAGAGAAACAAAAAAACTATCCCAAAGCAAAGT
TGCGAGTAAAGTGGGTATTCGGCAAGATACTGTCTCCAATTTTGAACTTTCTCCAGACTCTACCAAATTAGAAACCTTCT
TTAAGATTTTATCTGCATTAAATTTAGAACTCGATATTAAACCCAGAAATGAAAGTGGTTCATCAAACACAGACTCCGGA
TGGAAGGAAGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0L7D489