Detailed information of TA system TA239965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 5144001..5144979
• Replicon: chromosome
• Accession: NZ_CP094624
• Organism: Bacillus thuringiensis strain LX43

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MTX16_RS26410
• Protein ID: WP_243820993.1
• Coordinates: 5144001..5144738 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: MTX16_RS26415
• Protein ID: WP_000237818.1
• Coordinates: 5144851..5144979 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTX16_RS26375	5139138..5139382	+	245	Protein_5130	hypothetical protein	-
MTX16_RS26380	5139482..5140192	+	711	WP_243820992.1	class I SAM-dependent methyltransferase	-
MTX16_RS26385	5140310..5140717	+	408	WP_000063589.1	VOC family protein	-
MTX16_RS26390	5140730..5141119	+	390	Protein_5133	SAM-dependent methyltransferase	-
MTX16_RS26395	5141277..5142268	-	992	Protein_5134	thiamine pyrophosphate-dependent enzyme	-
MTX16_RS26400	5142376..5142858	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
MTX16_RS26405	5143025..5143762	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
MTX16_RS26410	5144001..5144738	+	738	WP_243820993.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MTX16_RS26415	5144851..5144979	+	129	WP_000237818.1	hypothetical protein	Antitoxin
MTX16_RS26420	5145052..5145228	+	177	WP_000852629.1	stage II sporulation protein SB	-
MTX16_RS26425	5145247..5145636	-	390	WP_243820994.1	YxeA family protein	-
MTX16_RS26430	5145848..5147317	+	1470	WP_243820995.1	beta-Ala-His dipeptidase	-
MTX16_RS26435	5147563..5148372	+	810	WP_000664546.1	papain-like cysteine protease family protein	-
MTX16_RS26440	5148398..5149000	+	603	WP_098906237.1	hypothetical protein	-
MTX16_RS26445	5149244..5149495	-	252	WP_000827560.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28319.09 Da
• Isoelectric Point: 8.2998

>T239965 WP_243820993.1 NZ_CP094624:5144001-5144738 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLILWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T239965 NZ_LS483422:1477434-1477844 [Providencia heimbachae]
GTGGTCCTGTGGAAATCTAATCTCGCCATTTCATGGAAAACTCAACTTTTTTCTACCTGTATTCATGGGGTAATAGGCGC
ATTTGTGCTAATTGCTCCGTGGGTATCAGAAACCTCTATGATTTGGCTGCCATTGCTGGTGGTGATTGTCGCGAGTTGGG
CGAAAAGCCAGAAAAATATCAGTAAAATTAAAGGGATCGTTGTTCTGGTTAACGGCAATAAAGTTCAGTGGAAGAAAAAT
GAGTGGTATATTGATAAAGCGCCTTGGCGCAGTCGATTTGGTATTTTGCTGACGCTAAGTGCCCTACAAGGCAAGCCGAA
AAAAATTCATTTGTGGATTGCGAAAGATACAGTTTCAGAAGAAAATTGGCGAAATTTAAATCAGCTATTACTGCAATATC
CAGATATTTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT239965 WP_000237818.1 NZ_CP094624:5144851-5144979 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT239965 NZ_LS483422:1477187-1477453 [Providencia heimbachae]
ATGGATATAGAGAATAAAGCGCGAATTCATTGGGCTTGTAGACGTGGTATGCGTGAATTGGATATTTCAATCATGCCTTT
TTTTGAATATGAGTATGATGGTTTATCGAGTGAAGACAAGCAGACGTTTGTGAATCTGTTGGAATGTGCTGATCCAGACT
TATTCAACTGGTTAATGAATCATGGTCGTCCAGATGACGATAAATTATTTTATATGGTAAAACTGATTCAGGAAAAAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Structures

Antitoxin

• AlphaFold DB: W8YZL5